Počet záznamů: 1
Antiviral activity of the adenosine analogue BCX4430 against West Nile virus and tick-borne flaviviruses
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SYSNO ASEP 0479053 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antiviral activity of the adenosine analogue BCX4430 against West Nile virus and tick-borne flaviviruses Tvůrce(i) Eyer, Luděk (BC-A) RID, ORCID
Zouharová, D. (CZ)
Širmarová, J. (CZ)
Fojtiková, M. (CZ)
Štefánik, M. (CZ)
Haviernik, J. (CZ)
Nencka, Radim (UOCHB-X) RID, ORCID
De Clercq, E. (BE)
Růžek, Daniel (BC-A) RID, ORCIDCelkový počet autorů 9 Zdroj.dok. Antiviral Research. - : Elsevier - ISSN 0166-3542
Roč. 142, JUN (2017), s. 63-67Poč.str. 5 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova BCX4430 ; Flavivirus ; adenosine analogue ; nucleoside inhibitor ; antiviral activity ; cytotoxicity Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Virology CEP NV16-34238A GA MZd - Ministerstvo zdravotnictví GA16-20054S GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 ; UOCHB-X - RVO:61388963 UT WOS 000401203100008 EID SCOPUS 85015984701 DOI 10.1016/j.antiviral.2017.03.012 Anotace There are currently no approved antiviral therapies against medically important human flaviviruses. The imino-C-nucleoside BCX4430 shows broad-spectrum antiviral activity against a wide range of RNA viruses. Here, we demonstrate that BCX4430 inhibits tick-borne species of the genus Flavivirus, however, the antiviral effect varies against individual species. Micro-molar BCX4430 levels inhibited tick-borne encephalitis virus (TBEV), while, approximately 3-8-fold higher concentrations were needed to inhibit louping ill virus and Kyasanur Forest disease virus. Moreover, the compound strongly inhibited in vitro replication of West Nile virus, a typical mosquito-transmitted flavivirus. Two chemical forms of the compound, i.e. BCX4430 and BCX4430 hydrochloride, were compared and both exerted similar inhibitory profiles in our in vitro antiviral assay systems and no or negligible cytotoxicity in porcine kidney stable and Vero cells. The obtained data indicate that, in addition to mosquito-borne flaviviruses, the compound has strong antiviral activity against members of the TBEV serocomplex. (C) 2017 Elsevier B.V. All rights reserved. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1