Počet záznamů: 1
Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies
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SYSNO ASEP 0455666 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of the expression of sperm proteins in normozoospermic and asthenozoospermic men using monoclonal antibodies Tvůrce(i) Čapková, Jana (BTO-N)
Kubátová, Alena (BTO-N) RID
Děd, Lukáš (BTO-N) RID
Teplá, O. (CZ)
Pěknicová, Jana (BTO-N) RIDCelkový počet autorů 5 Zdroj.dok. Asian Journal of Andrology - ISSN 1008-682X
Roč. 18, č. 1 (2016), s. 108-113Poč.str. 6 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. IN - Indie Klíč. slova asthenozoospermia ; flow cytometry ; fluorescence microscopy ; monoclonal antibodies ; sperm proteins Vědní obor RIV CE - Biochemie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP503/12/1834 GA ČR - Grantová agentura ČR Institucionální podpora BTO-N - RVO:86652036 UT WOS 000367846900018 DOI 10.4103/1008-682X.151400 Anotace Recent studies have shown that infertility affects estimated 15% of all couples. Male infertility is the primary or contributory cause in 60% of these cases. Consequently, the application of assisted reproduction is increasing. These methods could benefit from an extended evaluation of sperm quality. For this reason, we analyzed sperm proteins from 30 men with normal spermiograms and 30 men with asthenozoospermia. Ejaculates of both groups were tested by flow cytometry (FCM) and fluorescence with a set of well-characterized anti-human sperm Hs-monoclonal antibodies (MoAbs), which were generated in our laboratory. No statistically significant differences were found between normospermics and asthenospermics in the expression of the sperm surface protein clusterin, evaluated with Hs-3 MoAb, and semenogelin, evaluated with Hs-9 MoAb. However, FCM revealed quantitative differences in the acrosomal proteins between normozoospermic and asthenozoospermic men, namely, in glyceraldehyde-3-phosphate dehydrogenase, evaluated with Hs-8 MoAb, valosin-containing protein, evaluated with Hs-14 MoAb, and ATP synthase (cAMP-dependent protein kinase II, PRKAR2A), evaluated with MoAb Hs-36. Asthenozoospermic men displayed a highly reduced expression of intra-acrosomal proteins, with a likely decrease in sperm quality, and thus a negative impact on successful reproduction. Asthenozoospermia seems to be a complex disorder involving intra-acrosomal proteins. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2016
Počet záznamů: 1