Počet záznamů: 1
Transstadial Transmission of Borrelia turcica in Hyalomma aegyptium Ticks
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SYSNO ASEP 0453379 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transstadial Transmission of Borrelia turcica in Hyalomma aegyptium Ticks Tvůrce(i) Kalmár, Z. (RO)
Cozma, V. (RO)
Sprong, H. (NL)
Jahfari, S. (NL)
D’Amico, G. (RO)
Mărcutan, D.I. (RO)
Ionică, A.M. (RO)
Magdaş, C. (RO)
Modrý, David (BC-A) RID, ORCID
Mihalca, A. D. (RO)Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 10, č. 2 (2015), e0115520Poč.str. 9 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Borne relapsing fever ; Lyme disease ; imported reptiles Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Vědní obor RIV – spolupráce Choroby a škůdci zvířat, veterinární medicína Institucionální podpora BC-A - RVO:60077344 UT WOS 000350062300005 DOI 10.1371/journal.pone.0115520 Anotace Borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylogenetically divergent from other Borrelia groups. The novel group of Borrelia was initially isolated from Hyalomma aegyptium ticks in Turkey and it was recently found in blood and multiple organs of tortoises exported from Jordan to Japan. However, the ecology of these spirochetes and their development in ticks or the vertebrate hosts were not investigated in detail; our aims were to isolate the pathogen and to evaluate the possibility of transstadial transmission of Borrelia turcica by H. aegyptium ticks. Ticks were collected from Testudo graeca tortoises during the summer of 2013 from southeastern Romania. Engorged nymphs were successfully molted to the adult stage. Alive B. turcica was isolated from molted ticks by using Barbour-Stoenner-Kelly (BSK) II medium. Four pure cultures of spirochetes were obtained and analyzed by PCR and sequencing. Sequence analysis of glpQ, gyrB and flaB revealed 98%-100% similarities with B. turcica. H. aegyptium ticks collected from T. graeca tortoises were able to pass the infection with B. turcica via transstadial route, suggesting its vectorial capacity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016
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