Počet záznamů: 1
Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae
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SYSNO ASEP 0451085 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae Tvůrce(i) Huang, T. (CN)
Xiao, Y. (CN)
Pan, J. (CN)
Zhang, L. (CN)
Gelbič, Ivan (BC-A) RID, ORCID
Guan, X. (CN)Celkový počet autorů 6 Zdroj.dok. Open Life Sciences. - : Walter de Gruyter - ISSN 2391-5412
Roč. 10, č. 1 (2015), s. 521-528Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova Bacillus thuringiensis subsp. galleriae ; PCR-RFLP ; cloning Vědní obor RIV EB - Genetika a molekulární biologie Institucionální podpora BC-A - RVO:60077344 UT WOS 000371475600027 EID SCOPUS 84948779036 DOI 10.1515/biol-2015-0054 Anotace Two cry1-type genes encoding insecticidal crystal proteins (ICPs) were detected by PCR-RFLP and cloned from Bacillus thuringiensis subsp. galleriae 87. The nukleotide sequences were deposited in GenBank with accession numbers EU679501 and EU679502, and designated as cry1Fb7 and cry1Cb3 respectively by B. thuringiensis Delta-Endotoxin Nomenclature Committee. cry1Cb3 shared 99% homology with other cry1Cb genes. The existence of two additional stop codons indicated cry1Cb3 was a silent gene. The cry1Cb3 was 3531 bp with 38.98% G+C content and its first open reading frame (ORF) encoded a protein of 213 amino acid residues with a calculated molecular weight of 23.8 kDa and a predicted pI value of 4.63. Five amino acid sequence blocks (block 1, block 2, block 3, block 4 and block 5) were found in Cry1Cb3. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2016 Elektronická adresa http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0054/biol-2015-0054.xml
Počet záznamů: 1