Počet záznamů: 1
Di- and tri-oxalkyl derivatives of a boron dipyrromethene (BODIPY) rotor dye in lipid bilayers
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SYSNO ASEP 0427760 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Di- and tri-oxalkyl derivatives of a boron dipyrromethene (BODIPY) rotor dye in lipid bilayers Tvůrce(i) Olšinová, Marie (UFCH-W) RID
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Pozník, M. (CZ)
Šachl, Radek (UFCH-W) RID, ORCID
Prausová, T. (CZ)
Hof, Martin (UFCH-W) RID, ORCID
Kozmik, V. (CZ)
Teplý, Filip (UOCHB-X) RID, ORCID
Svoboda, J. (CZ)
Cebecauer, Marek (UFCH-W) RID, ORCID, SAIZdroj.dok. Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076
Roč. 16, č. 22 (2014), s. 10688-10697Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova PLASMA-MEMBRANE VESICLES ; MOLECULAR ROTOR ; FLUORESCENCE POLARIZATION Vědní obor RIV CF - Fyzikální chemie a teoretická chemie CEP GAP305/11/0459 GA ČR - Grantová agentura ČR Institucionální podpora UFCH-W - RVO:61388955 ; UOCHB-X - RVO:61388963 UT WOS 000336781500048 EID SCOPUS 84900797647 DOI 10.1039/c4cp00888j Anotace The environment-sensitive fluorescent probes provide excellent tools for studying membranes in their native state. We have modified the BODIPY-based fluorescent molecular rotor by increasing the number of alkyl moieties from one to two or three to achieve a more defined and deeper positioning of the probe in membranes. Detailed characterisation of fluorescence properties and localisation/orientation of probes was performed using a variety of fluorescence techniques and model membranes composed of different lipids. As expected, additional alkyls attached to the fluorophore moiety led to a deeper and more defined localisation of the probe in the lipid bilayer. The results strongly indicate that fluorescence properties of such probes are influenced not only by lipid packing but also by the orientation of the probe in membranes. The orientation of rotors studied herein was significantly altered by changes in the lipid composition of membranes. Our observations demonstrate the limits of BODIPY-based molecular rotors as environmental sensors in cellular membranes with complex lipid composition. The results presented herein also underline the importance of the detailed characterisation of fluorescent membrane dyes and provide a guide for future testing. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2015
Počet záznamů: 1