Počet záznamů: 1
Raman microspectroscopy of algal lipid bodies: beta-carotene quantification
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SYSNO ASEP 0383732 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Raman microspectroscopy of algal lipid bodies: beta-carotene quantification Tvůrce(i) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Nedbal, Ladislav (UEK-B)
Trtílek, M. (CZ)Celkový počet autorů 8 Zdroj.dok. Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 24, č. 3 (2012), s. 541-546Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Raman microspectroscopy ; Microalgae ; Trachydiscus minutus ; Biotechnology ; Carotenoids Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika CEP FR-TI1/433 GA MPO - Ministerstvo průmyslu a obchodu ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 ; RVO:67179843 - RVO:67179843 UT WOS 000304187100029 DOI 10.1007/s10811-011-9754-4 Anotace Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of beta-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain beta-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of beta-carotene in a mixture of algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of beta-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that beta-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on the spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2013
Počet záznamů: 1