Počet záznamů: 1
Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots
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SYSNO ASEP 0172367 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Effect of 2-aminoindan-2-phosphonic acid on cell cycle progression in synchronous meristematic cells of Vicia faba roots Tvůrce(i) Cvikrová, Milena (UEB-Q) RID, ORCID
Binarová, Pavla (UEB-Q) RID
Cenklová, Věra (UEB-Q)
Eder, Josef (UEB-Q)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Macháčková, Ivana (UEB-Q)Zdroj.dok. Plant Science Bulletin. - : Botanical Society of America - ISSN 0032-0919
Roč. 164, č. 5 (2003), s. 823-832Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova cell cycle ; polyamines Vědní obor RIV CE - Biochemie CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5038910 - UEB-Q Anotace 2-Aminoindan-2-phosphonic acid (AIP), a potent inhibitor of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), was applied to highly synchronous meristematic cells of Vicia faba roots containing cells in G1/S and G2/M cell cycle checkpoints. The inhibition resulted in a slight decline of the content of hydroxycinnamic derivatives 3.5 h after the AIP application. Flow cytometric analyses and BrdU immunolabelling of replicated cells showed that AIP applied in the G1-phase of the cell cycle delayed the progression through S-phase. The transient delay in DNA replication in treated cells was accompanied by accumulation of free spermidine and perchloric acid (PCA)-soluble spermidine conjugates. The cell cycle checkpoint G2/M appeared to be less sensitive to the inhibitor than G1/S. Mitotic activity of asynchronous root meristems was decreased only after the highest tested AIP concentration was applied (100 M) for 24 h. Significant increase in free putrescine and perchloric acid soluble-putrescine conjugates in cells after AIP-treatment might represent stress response in cells after AIP application. Increased PA conjugation suggests that it has an important role in the control of free PA contents in cells. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2004
Počet záznamů: 1