Počet záznamů: 1
Bacterial nanotubes as a manifestation of cell death
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SYSNO ASEP 0534616 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bacterial nanotubes as a manifestation of cell death Tvůrce(i) Pospíšil, Jiří (MBU-M) ORCID, RID
Vítovská, Dragana (MBU-M) ORCID
Kofroňová, Olga (MBU-M) RID, ORCID
Muchová, K. (SK)
Šanderová, Hana (MBU-M) RID, ORCID
Hubálek, Martin (UOCHB-X) RID, ORCID
Šiková, Michaela (MBU-M) ORCID
Modrák, Martin (MBU-M) ORCID
Benada, Oldřich (MBU-M) ORCID, RID
Barák, I. (SK)
Krásný, Libor (MBU-M) RID, ORCIDČíslo článku 4963 Zdroj.dok. Nature Communications. - : Nature Publishing Group - ISSN 2041-1723
Roč. 11, č. 1 (2020)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova outer-membrane vesicles ; bacillus-subtilis ; peptidoglycan ; competence ; expression ; flagella ; reveals Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Ústav organické chemie a biochemie - Analytická chemie, separace CEP GA19-12956S GA ČR - Grantová agentura ČR LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV17-29680A GA MZd - Ministerstvo zdravotnictví Výzkumná infrastruktura ELIXIR-CZ - 90047 - Ústav organické chemie a biochemie AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 UT WOS 000577122000001 EID SCOPUS 85091873399 DOI https://doi.org/10.1038/s41467-020-18800-2 Anotace Bacterial nanotubes are membranous structures that have been reported to function as conduits between cells to exchange DNA, proteins, and nutrients. Here, we investigate the morphology and formation of bacterial nanotubes using Bacillus subtilis. We show that nanotube formation is associated with stress conditions, and is highly sensitive to the cells' genetic background, growth phase, and sample preparation methods. Remarkably, nanotubes appear to be extruded exclusively from dying cells, likely as a result of biophysical forces. Their emergence is extremely fast, occurring within seconds by cannibalizing the cell membrane. Subsequent experiments reveal that cell-to-cell transfer of non-conjugative plasmids depends strictly on the competence system of the cell, and not on nanotube formation. Our study thus supports the notion that bacterial nanotubes are a post mortem phenomenon involved in cell disintegration, and are unlikely to be involved in cytoplasmic content exchange between live cells. Bacterial nanotubes and other similar membranous structures have been reported to function as conduits between cells to exchange DNA, proteins, and nutrients. Here the authors provide evidence that bacterial nanotubes are formed only by dead or dying cells, thus questioning their previously proposed functions. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.nature.com/articles/s41467-020-18800-2
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