Počet záznamů: 1
UHPLC-MS/MS based target profiling of stress-induced phytohormones
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SYSNO ASEP 0433242 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název UHPLC-MS/MS based target profiling of stress-induced phytohormones Tvůrce(i) Floková, Kristýna (UEB-Q) ORCID, RID
Tarkowská, Danuše (UEB-Q) RID, ORCID
Miersch, Otto (UEB-Q) RID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Wasternack, Claus (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAIZdroj.dok. Phytochemistry. - : Elsevier - ISSN 0031-9422
Roč. 105, SEP 2014 (2014), s. 147-157Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Stress-induced phytohormones ; Jasmonates ; Abscisic acid Vědní obor RIV CB - Analytická chemie, separace CEP GA14-34792S GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LK21306 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000340986900019 DOI 10.1016/j.phytochem.2014.05.015 Anotace Stress-induced changes in phytohormone metabolite profiles have rapid effects on plant metabolic activity and growth. The jasmonates (JAs) are a group of fatty acid-derived stress response regulators with roles in numerous developmental processes. To elucidate their dual regulatory effects, which overlap with those of other important defence-signalling plant hormones such as salicylic acid (SA), abscisic acid (ABA) and indole-3-acetic acid (IAA), we have developed a highly efficient single-step clean-up procedure for their enrichment from complex plant matrices that enables their sensitive quantitative analysis using hyphenated mass spectrometry technique. The rapid extraction of minute quantities of plant material (less than 20 mg fresh weight. FW) into cold 10% methanol followed by one-step reversed-phase polymer-based solid phase extraction significantly reduced matrix effects and increased the recovery of labile JA analytes. This extraction and purification protocol was paired with a highly sensitive and validated ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method and used to simultaneously profile sixteen stress-induced phytohormones in minute plant material samples, including endogenous JA, several of its biosynthetic precursors and derivatives, as well as SA, ABA and IAA. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2015
Počet záznamů: 1