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Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination
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SYSNO ASEP 0601846 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination Tvůrce(i) Výmola, P. (CZ)
Garcia-Borja, E. (CZ)
Červenka, Jakub (UZFG-Y) ORCID
Balážiová, E. (CZ)
Vymolová, B. (CZ)
Vepřková, J. (CZ)
Vodička, Petr (UZFG-Y) ORCID
Skalníková, Helena (UZFG-Y) RID, ORCID
Tomáš, R. (CZ)
Netuka, D. (CZ)
Bušek, P. (CZ)
Šedo, A. (CZ)Číslo článku 13265 Zdroj.dok. Brain Pathology - ISSN 1015-6305
Roč. 34, č. 6 (2024)Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova collagen type I ; extracellular matrix proteins ; fibronectin ; glioblastoma ; pericytes ; proteomics Obor OECD Biochemistry and molecular biology CEP LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2023053 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001214406200001 EID SCOPUS 85192168720 DOI https://doi.org/10.1111/bpa.13265 Anotace Gliomagenesis induces profound changes in the composition of the extracellular matrix (ECM) of the brain. In this study, we identified a cellular population responsible for the increased deposition of collagen I and fibronectin in glioblastoma. Elevated levels of the fibrillar proteins collagen I and fibronectin were associated with the expression of fibroblast activation protein (FAP), which is predominantly found in pericyte-like cells in glioblastoma. FAP+ pericyte-like cells were present in regions rich in collagen I and fibronectin in biopsy material and produced substantially more collagen I and fibronectin in vitro compared to other cell types found in the GBM microenvironment. Using mass spectrometry, we demonstrated that 3D matrices produced by FAP+ pericyte-like cells are rich in collagen I and fibronectin and contain several basement membrane proteins. This expression pattern differed markedly from glioma cells. Finally, we have shown that ECM produced by FAP+ pericyte-like cells enhances the migration of glioma cells including glioma stem-like cells, promotes their adhesion, and activates focal adhesion kinase (FAK) signaling. Taken together, our findings establish FAP+ pericyte-like cells as crucial producers of a complex ECM rich in collagen I and fibronectin, facilitating the dissemination of glioma cells through FAK activation. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2025 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1111/bpa.13265
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