Počet záznamů: 1
Growth and mortality of aerobic anoxygenic phototrophs in the North Pacific Subtropical Gyre
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SYSNO ASEP 0585423 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Growth and mortality of aerobic anoxygenic phototrophs in the North Pacific Subtropical Gyre Tvůrce(i) Koblížek, Michal (MBU-M) RID, ORCID
Ferrera, I. (ES)
Kolářová, Eva (MBU-M)
Duhamel, S. (US)
Popendorf, K. J. (US)
Gasol, J. M. (ES)
Van Mooy, B. A. S. (US)Číslo článku e0003224 Zdroj.dok. Applied and Environmental Microbiology. - : American Society for Microbiology - ISSN 0099-2240
Roč. 90, č. 4 (2024)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova photoheterotrophic bacteria ; photosynthetic bacterium ; oligotrophic gradient ; surface waters ; light ; diversity ; rates ; bacteriochlorophyll ; variability ; phosphorus ; aerobic anoxygenic phototrophs ; bacteriochlorophyll a ; marine bacteria ; North Pacific Subtropical Gyre ; Station ALOHA Obor OECD Microbiology CEP GX19-28778X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 001193248200001 DOI 10.1128/aem.00032-24 Anotace Aerobic anoxygenic phototrophic (AAP) bacteria harvest light energy using bacteriochlorophyll-containing reaction centers to supplement their mostly heterotrophic metabolism. While their abundance and growth have been intensively studied in coastal environments, much less is known about their activity in oligotrophic open ocean regions. Therefore, we combined in situ sampling in the North Pacific Subtropical Gyre, north of O'ahu island, Hawaii, with two manipulation experiments. Infra-red epifluorescence microscopy documented that AAP bacteria represented approximately 2% of total bacteria in the euphotic zone with the maximum abundance in the upper 50 m. They conducted active photosynthetic electron transport with maximum rates up to 50 electrons per reaction center per second. The in situ decline of bacteriochlorophyll concentration over the daylight period, an estimate of loss rates due to predation, indicated that the AAP bacteria in the upper 50 m of the water column turned over at rates of 0.75-0.90 d-1. This corresponded well with the specific growth rate determined in dilution experiments where AAP bacteria grew at a rate 1.05 +/- 0.09 d-1. An amendment of inorganic nitrogen to obtain N:P = 32 resulted in a more than 10 times increase in AAP abundance over 6 days. The presented data document that AAP bacteria are an active part of the bacterioplankton community in the oligotrophic North Pacific Subtropical Gyre and that their growth was mostly controlled by nitrogen availability and grazing pressure. IMPORTANCE Marine bacteria represent a complex assembly of species with different physiology, metabolism, and substrate preferences. We focus on a specific functional group of marine bacteria called aerobic anoxygenic phototrophs. These photoheterotrophic organisms require organic carbon substrates for growth, but they can also supplement their metabolic needs with light energy captured by bacteriochlorophyll. These bacteria have been intensively studied in coastal regions, but rather less is known about their distribution, growth, and mortality in the oligotrophic open ocean. Therefore, we conducted a suite of measurements in the North Pacific Subtropical Gyre to determine the distribution of these organisms in the water column and their growth and mortality rates. A nutrient amendment experiment showed that aerobic anoxygenic phototrophs were limited by inorganic nitrogen. Despite this, they grew more rapidly than average heterotrophic bacteria, but their growth was balanced by intense grazing pressure. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2025 Elektronická adresa https://journals.asm.org/doi/10.1128/aem.00032-24
Počet záznamů: 1