Počet záznamů: 1
Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development
- 1.
SYSNO ASEP 0583614 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Endogenously produced hyaluronan contributes to the regulation of peritoneal adhesion development Tvůrce(i) Kocurková, Anna (BFU-R) ORCID
Kerberová, Michaela (BFU-R)
Nešporová, K. (CZ)
Lehka, K. (CZ)
Sandanusová, Miriam (BFU-R)
Simek, M. (CZ)
Velebný, V. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Ambrožová, Gabriela (BFU-R) RIDCelkový počet autorů 9 Zdroj.dok. Biofactors. - : Wiley - ISSN 0951-6433
Roč. 49, č. 4 (2023), s. 940-955Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova mesothelial cells ; involvement ; glycolysis ; activation ; prevention ; induction ; fibrosis Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000983044700001 EID SCOPUS 85158095023 DOI 10.1002/biof.1957 Anotace Peritoneal adhesions are postsurgical fibrotic complications connected to peritoneal inflammation. The exact mechanism of development is unknown, however, an important role is attributed to activated mesothelial cells (MCs) overproducing macromolecules of extracellular matrix (ECM), including hyaluronic acid (HA). It was suggested that endogenously-produced HA contributes to the regulation of different fibrosis-related pathologies. However, little is known about the role of altered HA production in peritoneal fibrosis. We focused on the consequences of the increased turnover of HA in the murine model of peritoneal adhesions. Changes of HA metabolism were observed in early phases of peritoneal adhesion development in vivo. To study the mechanism, human MCs MeT-5A and murine MCs isolated from the peritoneum of healthy mice were pro-fibrotically activated by transforming growth factor beta (TGF beta), and the production of HA was attenuated by two modulators of carbohydrate metabolism, 4-methylumbelliferone (4-MU) and 2-deoxyglucose (2-DG). The attenuation of HA production was mediated by upregulation of HAS2 and downregulation of HYAL2 and connected to the lower expression of pro-fibrotic markers, including fibronectin and a-smooth muscle actin (alpha SMA). Moreover, the inclination of MCs to form fibrotic clusters was also downregulated, particularly in 2-DG-treated cells. The effects of 2-DG, but not 4-MU, were connected to changes in cellular metabolism. Importantly, the inhibition of AKT phosphorylation was observed after the use of both HA production inhibitors. In summary, we identified endogenous HA as an important Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2024 Elektronická adresa https://iubmb.onlinelibrary.wiley.com/doi/10.1002/biof.1957
Počet záznamů: 1