Počet záznamů: 1
RNA localization during early development of the axolotl
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SYSNO ASEP 0583145 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název RNA localization during early development of the axolotl Tvůrce(i) Šimková, Kateřina (BTO-N)
Naraine, Ravindra (BTO-N)
Vintr, J. (CZ)
Soukup, V. (CZ)
Šindelka, Radek (BTO-N) RIDCelkový počet autorů 5 Číslo článku 1260795 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 11, OCT 19 2023 (2023)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova maternal messenger-rna ; germ plasm component ; vegetal cortex ; mitochondrial cloud ; animal-vegetal axis Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP GA20-23836S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 001095794600001 DOI 10.3389/fcell.2023.1260795 Anotace The asymmetric localization of biomolecules is critical for body plan development. One of the most popular model organisms for early embryogenesis studies is Xenopus laevis but there is a lack of information in other animal species. Here, we compared the early development of two amphibian species-the frog X. laevis and the axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along the animal-vegetal axis during the early development of A. mexicanum. For that purpose, we performed spatial transcriptome-wide analysis at low resolution, which revealed dynamic changes along the animal-vegetal axis classified into the following categories: profile alteration, de novo synthesis and degradation. Surprisingly, our results showed that many of the vegetally localized genes, which are important for germ cell development, are degraded during early development. Furthermore, we assessed the motif presence in UTRs of degraded mRNAs and revealed the enrichment of several motifs in RNAs of germ cell markers. Our results suggest novel reorganization of the transcriptome during embryogenesis of A. mexicanum to converge to the similar developmental pattern as the X. laevis. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2024 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2023.1260795/full
Počet záznamů: 1