Počet záznamů: 1
Transcriptional Inflammatory Signature in Healthy Donors and Different Radiotherapy Cancer Patients
- 1.0582663 - ÚJF 2025 RIV CH eng J - Článek v odborném periodiku
O'Brien, G. - Kamuda, M. - Cruz-Garcia, L. - Polozova, M. - Tichý, A. - Marková, M. - Širák, I. - Zahradníček, Oldřich - Widlak, P. - Ponge, L. - Polanska, J. - Badie, C.
Transcriptional Inflammatory Signature in Healthy Donors and Different Radiotherapy Cancer Patients.
International Journal of Molecular Sciences. Roč. 25, č. 2 (2024), č. článku 1080. ISSN 1661-6596
Institucionální podpora: RVO:61389005
Klíčová slova: inflammation * gene expression * biomarkers * human blood * cancer patients * radiotherapy
Obor OECD: Radiology, nuclear medicine and medical imaging
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://doi.org/10.3390/ijms25021080
Cancer and ionizing radiation exposure are associated with inflammation. To identify a set of radiation-specific signatures of inflammation-associated genes in the blood of partially exposed radiotherapy patients, differential expression of 249 inflammatory genes was analyzed in blood samples from cancer patients and healthy individuals. The gene expression analysis on a cohort of 63 cancer patients (endometrial, head and neck, and prostate cancer) before and during radiotherapy (24 h, 48 h, similar to 1 week, similar to 4-8 weeks, and 1 month after the last fraction) identified 31 genes and 15 up- and 16 down-regulated genes. Transcription variability under normal conditions was determined using blood drawn on three separate occasions from four healthy donors. No difference in inflammatory expression between healthy donors and cancer patients could be detected prior to radiotherapy. Remarkably, repeated sampling of healthy donors revealed an individual endogenous inflammatory signature. Next, the potential confounding effect of concomitant inflammation was studied in the blood of seven healthy donors taken before and 24 h after a flu vaccine or ex vivo LPS (lipopolysaccharide) treatment. Flu vaccination was not detected at the transcriptional level and LPS did not have any effect on the radiation-induced signature identified. Finally, we identified a radiation-specific signature of 31 genes in the blood of radiotherapy patients that were common for all cancers, regardless of the immune status of patients. Confirmation via MQRT-PCR was obtained for BCL6, MYD88, MYC, IL7, CCR4 and CCR7. This study offers the foundation for future research on biomarkers of radiation exposure, radiation sensitivity, and radiation toxicity for personalized radiotherapy treatment.
Trvalý link: https://hdl.handle.net/11104/0350742
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