Počet záznamů: 1
Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans
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SYSNO ASEP 0582381 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in Caenorhabditis elegans Tvůrce(i) Panska, L. (CZ)
Nedvedova, S. (CZ)
Vacek, V. (CZ)
Křivská, D. (CZ)
Konečný, L. (CZ)
Knop, F. (CZ)
Kutil, Zsofia (BTO-N) RID, ORCID
Škultétyová, Ĺubica (BTO-N) RID
Leontovyč, Adrian (UOCHB-X) ORCID
Ulrychová, Lenka (UOCHB-X) ORCID, RID
Sakanari, J. (US)
Asahina, M. (US)
Bařinka, Cyril (BTO-N) RID, ORCID
Macurkova, M. (CZ)
Dvořák, Jan (UOCHB-X) ORCIDCelkový počet autorů 15 Číslo článku BSR20230502 Zdroj.dok. Bioscience Reports. - : Portland Press - ISSN 0144-8463
Roč. 44, č. 1 (2024)Poč.str. 21 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova amyotrophic-lateral-sclerosis ; c-terminal domain ; membrane antigen ; n-acetylaspartylglutamate Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA18-14167S GA ČR - Grantová agentura ČR Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i.Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 ; UOCHB-X - RVO:61388963 UT WOS 001147679800001 EID SCOPUS 85182501686 DOI 10.1042/BSR20230502 Anotace Human glutamate carboxypeptidase 2 (GCP2) from the M28B metalloprotease group is an important target for therapy in neurological disorders and an established tumor marker. However, its physiological functions remain unclear. To better understand general roles, we used the model organism Caenorhabditis elegans to genetically manipulate its three existing orthologous genes and evaluate the impact on worm physiology. The results of gene knockout studies showed that C. elegans GCP2 orthologs affect the pharyngeal physiology, reproduction, and structural integrity of the organism. Promoter-driven GFP expression revealed distinct localization for each of the three gene paralogs, with gcp-2.1 being most abundant in muscles, intestine, and pharyngeal interneurons, gcp-2.2 restricted to the phasmid neurons, and gcp-2.3 located in the excretory cell. The present study provides new insight into the unique phenotypic effects of GCP2 gene knockouts in C. elegans, and the specific tissue localizations. We believe that elucidation of particular roles in a non-mammalian organism can help to explain important questions linked to physiology of this protease group and in extension to human GCP2 involvement in pathophysiological processes. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2024 Elektronická adresa https://portlandpress.com/bioscirep/article/44/1/BSR20230502/233851/Uncovering-the-essential-roles-of-glutamate
Počet záznamů: 1