Počet záznamů: 1
Culture-dependent screening of endospore-forming clostridia in infant feces
- 1.
SYSNO ASEP 0581028 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Culture-dependent screening of endospore-forming clostridia in infant feces Tvůrce(i) Ingribelli, E. (CZ)
Modráčková, N. (CZ)
Tejnecký, V. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Schwab, C. (CH)
Neužil-Bunešová, V. (CZ)Celkový počet autorů 6 Číslo článku 347 Zdroj.dok. BMC Microbiology. - : BioMed Central - ISSN 1471-2180
Roč. 23, č. 1 (2023)Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Infant gut microbiota ; Cultivation ; Endospore formers ; Clostridium ; Fermentation profiles ; Butyrate Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001107264700007 EID SCOPUS 85176925800 DOI https://doi.org/10.1186/s12866-023-03104-4 Anotace Background Only a few studies dealt with the occurrence of endospore-forming clostridia in the microbiota of infants without obvious health complications.Methods A methodology pipeline was developed to determine the occurrence of endospore formers in infant feces. Twenty-four fecal samples (FS) were collected from one infant in monthly intervals and were subjected to variable chemical and heat treatment in combination with culture-dependent analysis. Isolates were identified by MALDI-TOF mass spectrometry, 16S rRNA gene sequencing, and characterized with biochemical assays.Results More than 800 isolates were obtained, and a total of 21 Eubacteriales taxa belonging to the Clostridiaceae, Lachnospiraceae, Oscillospiraceae, and Peptostreptococcaceae families were detected. Clostridium perfringens, C. paraputrificum, C. tertium, C. symbiosum, C. butyricum, and C. ramosum were the most frequently identified species compared to the rarely detected Enterocloster bolteae, C. baratii, and C. jeddahense. Furthermore, the methodology enabled the subsequent cultivation of less frequently detectable gut taxa such as Flavonifractor plautii, Intestinibacter bartlettii, Eisenbergiella tayi, and Eubacterium tenue. The isolates showed phenotypic variability regarding enzymatic activity, fermentation profiles, and butyrate production.Conclusions Taken together, this approach suggests and challenges a cultivation-based pipeline that allows the investigation of the population of endospore formers in complex ecosystems such as the human gastrointestinal tract. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2024 Elektronická adresa https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03104-4
Počet záznamů: 1