Počet záznamů: 1
First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics
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SYSNO ASEP 0580535 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název First insight into strongylid nematode diversity and anthelmintic treatment effectiveness in beef cattle in the Czech Republic explored by HTS metagenomics Tvůrce(i) Pafčo, Barbora (UBO-W) RID, ORCID, SAI
Nosková, E. (CZ)
Ilík, V. (CZ)
Anettová, L. (CZ)
Červená, Barbora (UBO-W) RID, ORCID, SAI
Kreisinger, J. (CZ)
Pšenková, I. (CZ)
Václavek, P. (CZ)
Vyhlídalová, T. (CZ)
Ježková, J. (CZ)
Malát, K. (CZ)
Mihalca, A. D. (RO)
Modrý, David (BC-A) RID, ORCIDCelkový počet autorů 0 Číslo článku 100961 Zdroj.dok. Veterinary Parasitology: Regional Studies and Reports. - : Elsevier
Roč. 47, January (2024)Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Anthelmintic treatment ; Cattle ; Resistance ; Strongylid nematodes Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Veterinary science Výzkumná infrastruktura NCMG II - 90132 - Univerzita Karlova
e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osobZpůsob publikování Omezený přístup Institucionální podpora UBO-W - RVO:68081766 ; BC-A - RVO:60077344 EID SCOPUS 85179478065 DOI 10.1016/j.vprsr.2023.100961 Anotace Parasitic diseases and mitigation of their effects play an important role in the health management of grazing livestock worldwide, with gastrointestinal strongylid nematodes being of prominent importance. These helminths typically occur in complex communities, often composed of species from numerous strongylid genera. Detecting the full diversity of strongylid species in non-invasively collected faecal samples is nearly impossible using conventional methods. In contrast, high-throughput amplicon sequencing (HTS) can effectively identify co-occurring species. During the four-year project, we collected and analysed faecal samples from beef cattle on >120 farms throughout the Czech Republic. Strongylids were the predominant nematodes, detected in 56% of the samples, but at a low level of infection. The apparent limitations in identifying strongylid taxa prompted this pilot study on a representative group of samples testing positive for strongylids using ITS-2 metabarcoding. The most widespread genera parasitizing Czech cattle were Ostertagia (O. ostertagi) and Oesophagostomum spp., followed by Trichostrongylus and Cooperia, while Bunostomum, Nematodirus and Chabertia were present only in a minority. As comparative material, 21 samples of cattle from the Danube Delta in Romania were used, which, in contrast, were dominated by Haemonchus placei. Finally, the effect of ivermectin treatment was tested at two Czech farms. After treatment with the anthelmintic, there was a shift in the strongylid communities, with a dominance of Cooperia and Ostertagia. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2025 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2405939023001314?via%3Dihub
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