Počet záznamů: 1
Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: iIn vitro/i-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling
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SYSNO ASEP 0579877 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Unravelling heterogeneous effects of cancer-associated fibroblasts on poor prognosis markers in breast cancer EM-G3 cell line: iIn vitro/i-targeted treatment (anti-IL-6, anti-VEGF-A, anti-MFGE8) based on transcriptomic profiling Tvůrce(i) Urban, L. (SK)
Novák, S. (CZ)
Coma, M. (SK)
Dvořánková, B. (CZ)
Lacina, L. (CZ)
Šáchová, Jana (UMG-J)
Hradilová, Miluše (UMG-J)
Svatoňová, Petra (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Strnad, Hynek (UMG-J) RID
Březinová, J. (CZ)
Smetana, K. (CZ)
Gal, P. (SK)
Szabó, P. (SK)Celkový počet autorů 14 Číslo článku 3 Zdroj.dok. Oncology Reports - ISSN 1021-335X
Roč. 51, č. 1 (2024)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GR - Řecko Klíč. slova expression ; growth ; hallmarks ; diagnosis ; vimentin ; invasion ; epithelial-mesenchymal interaction ; cell differentiation ; tumor microenvironment ; breast cancer ; neutralizing antibody Obor OECD Cell biology CEP EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LX22NPO5102 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 001111760900001 EID SCOPUS 85177454254 DOI 10.3892/or.2023.8662 Anotace Breast cancer is the most frequently diagnosed cancer in women worldwide. Although dramatically increased survival rates of early diagnosed cases have been observed, late diagnosed patients and metastatic cancer may still be considered fatal. The present study's main focus was on cancer-associated fibroblasts (CAFs) which is an active component of the tumor microenvironment (TME) regulating the breast cancer ecosystem. Transcriptomic profiling and analysis of CAFs isolated from breast cancer skin metastasis, cutaneous basal cell carcinoma, and squamous cell carcinoma unravelled major gene candidates such as IL6, VEGFA and MFGE8 that induced co-expression of keratins-8/-14 in the EM-G3 cell line derived from infiltrating ductal breast carcinoma. Western blot analysis of selected keratins (keratin-8,14,18,19) and epithelial-mesenchymal transition-associated markers (SLUG, SNAIL, ZEB1, E-/N-cadherin, vimentin) revealed specific responses pointing to certain heterogeneity of the studied CAF populations. Experimental in vitro treatment using neutralizing antibodies against IL-6, VEGF-A and MFGE8 attenuated the modulatory effect of CAFs on EM-G3 cells. The present study provided novel data in characterizing and understanding the interactions between CAFs and EM-G3 cells in vitro. CAFs of different origins support the pro-inflammatory microenvironment and influence the biology of breast cancer cells. This observation potentially holds significant interest for the development of novel, clinically relevant approaches targeting the TME in breast cancer. Furthermore, its implications extend beyond breast cancer and have the potential to impact a wide range of other cancer types. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2025 Elektronická adresa https://www.spandidos-publications.com/10.3892/or.2023.8662
Počet záznamů: 1