Počet záznamů: 1
Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis
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SYSNO ASEP 0576970 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis Tvůrce(i) Krejčí, Jana (BFU-R) RID, ORCID
Arcidiacono, Orazio Angelo (BFU-R) ORCID
Čegan, Radim (BFU-R) RID, ORCID
Radaszkiewicz, K. A. (CZ)
Pacherník, J. (CZ)
Pirk, J. (CZ)
Pešl, M. (CZ)
Fila, P. (CZ)
Bártová, Eva (BFU-R) ORCIDCelkový počet autorů 9 Zdroj.dok. Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 72, č. 4 (2023), s. 425-444Poč.str. 2023 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova mES cells ; hES cells ; fto ; alkbh5 ; Epigenetics ; Differentiation ; Aging Vědní obor RIV ED - Fyziologie Obor OECD Biochemistry and molecular biology Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 001078316100002 EID SCOPUS 85173044274 DOI 10.33549/physiolres.935078 Anotace FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2024 Elektronická adresa chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.biomed.cas.cz/physiolres/pdf/2023/72_425.pdf
Počet záznamů: 1