Počet záznamů: 1
Synthetic and analytical routes to the L-amino acid conjugates of cis-OPDA and their identification and quantification in plants
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SYSNO ASEP 0576827 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Synthetic and analytical routes to the L-amino acid conjugates of cis-OPDA and their identification and quantification in plants Tvůrce(i) Mik, V. (CZ)
Pospíšil, T. (CZ)
Brunoni, Federica (UEB-Q) ORCID
Grúz, J. (CZ)
Nožková, V. (CZ)
Wasternack, Claus (UEB-Q) ORCID
Miersch, Otto (UEB-Q) RID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Floková, Kristýna (UEB-Q) ORCID, RID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Široká, Jitka (UEB-Q) ORCIDCelkový počet autorů 11 Číslo článku 113855 Zdroj.dok. Phytochemistry. - : Elsevier - ISSN 0031-9422
Roč. 215, NOV (2023)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Amino acid conjugates ; Arabidopsis thaliana (Brassicaceae) ; Cis-OPDA ; Identification ; Jasmonates ; Labeled compounds ; Liquid chromatography ; Tandem mass spectrometry ; Wounding Obor OECD Plant sciences, botany Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 001077363900001 EID SCOPUS 85171148358 DOI 10.1016/j.phytochem.2023.113855 Anotace Cis-(+)-12-oxophytodienoic acid (cis-(+)-OPDA) is a bioactive jasmonate, a precursor of jasmonic acid, which also displays signaling activity on its own. Modulation of cis-(+)-OPDA actions may be carried out via biotransformation leading to metabolites of various functions. This work introduces a methodology for the synthesis of racemic cis-OPDA conjugates with amino acids (OPDA-aa) and their deuterium-labeled analogs, which enables the unambiguous identification and accurate quantification of these compounds in plants. We have developed a highly sensitive liquid chromatography-tandem mass spectrometry-based method for the reliable determination of seven OPDA-aa (OPDA-Alanine, OPDA-Aspartate, OPDA-Glutamate, OPDA-Glycine, OPDA-Isoleucine, OPDA-Phenylalanine, and OPDA-Valine) from minute amount of plant material. The extraction from 10 mg of fresh plant tissue by 10% aqueous methanol followed by single-step sample clean-up on hydrophilic–lipophilic balanced columns prior to final analysis was optimized. The method was validated in terms of accuracy and precision, and the method parameters such as process efficiency, recovery and matrix effects were evaluated. In mechanically wounded 30-day-old Arabidopsis thaliana leaves, five endogenous (+)-OPDA-aa were identified and their endogenous levels were estimated. The time-course accumulation revealed a peak 60 min after the wounding, roughly corresponding to the accumulation of cis-(+)-OPDA. Our synthetic and analytical methodologies will support studies on cis-(+)-OPDA conjugation with amino acids and research into the biological significance of these metabolites in plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.phytochem.2023.113855
Počet záznamů: 1