Počet záznamů: 1
Independent loss events of a functional tetherin gene in galliform birds
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SYSNO ASEP 0576431 Druh ASEP J - Článek v odborném periodiku Zařazení RIV Záznam nebyl označen do RIV Poddruh J Článek ve WOS Název Independent loss events of a functional tetherin gene in galliform birds Tvůrce(i) Krchlíková, Veronika (UMG-J)
Lotke, R. (DE)
Haussmann, I. (DE)
Reinišová, Markéta (UMG-J)
Kučerová, Dana (UMG-J)
Pecnová, Lubomíra (UMG-J)
Ungrová, Lenka (UMG-J)
Hejnar, Jiří (UMG-J) RID
Sauter, D. (DE)
Elleder, Daniel (UMG-J) RIDCelkový počet autorů 10 Číslo článku e0080323 Zdroj.dok. Journal of Virology - ISSN 0022-538X
Roč. 97, č. 10 (2023)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova retroviruses ; restriction factors ; gene loss ; turkey ; tetherin Obor OECD Biochemistry and molecular biology CEP GA20-22063S GA ČR - Grantová agentura ČR LX22NPO5103 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 001068528200004 EID SCOPUS 85175823586 DOI 10.1128/jvi.00803-23 Anotace The restriction factor tetherin (bone marrow stromal cell antigen 2) is an interferon-inducible protein preventing the release of newly formed viral particles from infected cells. Tetherin displays antiviral activity against a broad range of enveloped viruses, including retroviruses. While tetherin orthologs have been identified in several mammalian species, little is known about its expression and activity in non-mammalian vertebrates, including birds. We have previously described antiviral activity of chicken (Gallus gallus) tetherin against the prototypical avian retrovirus avian sarcoma and leukosis virus (ASLV). Here, we report the loss of functional tetherin orthologs in several galliform birds, including turkey (Meleagris gallopavo) and Mikado pheasant (Syrmaticus mikado). In both species, the tetherin coding sequence acquired inactivating mutations, including an in-frame stop codon and frameshifting deletions. Similar to the chicken tetherin ortholog, reconstituted turkey and Mikado pheasant tetherins restricted ASLV and HIV-1 indicating antiviral activity of the ancestor protein. Previous work revealed the presence of the TMCC(aT) gene in close proximity to tetherin, encoding a protein with tetherin-like structure in multiple vertebrates. Intriguingly, ectopic overexpression of chicken and turkey TMCC(aT) proteins in human cells decreased total HIV-1 yield. In contrast to tetherin, however, TMCC(aT) did not specifically restrict virion release, suggesting distinct antiviral mechanisms. In line with this, IFNa stimulation did not reduce the release of ASLV particles from infected turkey cells. Overall, our data describe the loss of functional tetherin genes in several galliform species and identify an antiviral activity of the related TMCC(aT) protein that is mechanistically different from that of tetherin. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2024 Elektronická adresa https://journals.asm.org/doi/10.1128/jvi.00803-23
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