Počet záznamů: 1
Application of capillary electrophoresis in roughened fused silica capillary for classification of clinical Cutibacterium acnes isolates
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SYSNO ASEP 0576390 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Application of capillary electrophoresis in roughened fused silica capillary for classification of clinical Cutibacterium acnes isolates Tvůrce(i) Šalplachta, Jiří (UIACH-O) RID, ORCID
Horká, Marie (UIACH-O) RID, ORCID
Růžička, F. (CZ)
Karásek, Pavel (UIACH-O) RID, ORCID
Siváková, A. (CZ)
Roth, Michal (UIACH-O) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. ITP 2023. Abstracts. -, 2023
S. 142-142Poč.str. 1 s. Forma vydání Online - E Akce International Symposium on Electro- and Liquid-Phase Separation Techniques /29./ ITP 2023 Datum konání 13.09.2023 - 17.09.2023 Místo konání San Felice Circeo Země IT - Itálie Typ akce WRD Jazyk dok. eng - angličtina Země vyd. IT - Itálie Klíč. slova capillary electrophoresis ; classification ; cutibacterium acne ; fused silica capillary ; supercritical water Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP NU22-05-00110 GA MZd - Ministerstvo zdravotnictví Institucionální podpora UIACH-O - RVO:68081715 Anotace Cutibacterium acnes, commensal bacterium involved in the maintenance of healthy skin, is also associated with inflammatory diseases. Inflammatory and immunogenic properties vary between C. acnes phylotypes, therefore, reliable classification of clinical C. acnes isolates is important for determining their pathogenicity. In this study, a combination of capillary electrophoretic methods, polymer-enhanced transient sotachophoresis and sweeping of the charged bacterial cells in micellar electrokinetic chromatography in the roughened fused silica capillary, was used for the classification of clinical C. acnes isolates into the individual phylotypes. The analysis was done in twenty minutes at laboratory temperature. Separation of the individual isolates of some phylotype groups was achieved by decreasing the separation temperature to 15 °C. Relative standard deviations of migration times of both intra- and inter-day analyses did not exceed 1.7 % and the very good linearity of the proposed method in the concentration range from 5×105 to 1×107 cells mL-1 was achieved (coefficient of determination R2=0.9985). Limit of detection for all the examined bacteria was determined as 5×105 cells mL-1. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2024
Počet záznamů: 1