Development of recombinant West Nile virus expressing mCherry reporter protein

0574469 - BC 2024 RIV NL eng J - Článek v odborném periodiku
Kobayashi, S. - Fukuda, Y. - Yoshii, K. - Thammahakin, P. - Maezono, K. - Eyer, Luděk - Růžek, Daniel - Kariwa, H.
Development of recombinant West Nile virus expressing mCherry reporter protein.
Journal of Virological Methods. Roč. 317, APR (2023), č. článku 114744. ISSN 0166-0934. E-ISSN 1879-0984
Institucionální podpora: RVO:60077344
Klíčová slova: West Nile virus * Reporter protein * Production of reporter virus
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.1, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0166093423000691?via%3Dihub

West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.
Trvalý link: https://hdl.handle.net/11104/0345586