Počet záznamů: 1
Gut microbiota assembly of Gotland varroa-surviving honey bees excludes major viral pathogens
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SYSNO ASEP 0574248 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gut microbiota assembly of Gotland varroa-surviving honey bees excludes major viral pathogens Tvůrce(i) Svobodová, K. (CZ)
Maitre, A. (FR)
Obregón, D. (CA)
Wu-Chuang, A. (FR)
Thaduri, S. (SE)
Locke, B. (SE)
de Miranda, J. R. (SE)
Mateos-Hernández, L. (FR)
Bruce Krejčí, Alena (BC-A) RID, ORCID
Cabezas-Cruz, A. (FR)Celkový počet autorů 10 Číslo článku 127418 Zdroj.dok. Microbiological Research. - : Elsevier - ISSN 0944-5013
Roč. 274, SEP 01 (2023)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova varroa ; honey bees ; varroa-surviving Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Biochemistry and molecular biology Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 001030363900001 EID SCOPUS 85162005329 DOI 10.1016/j.micres.2023.127418 Anotace The spread of the parasite Varroa destructor and associated viruses has resulted in massive honey bee colony losses with considerable economic and ecological impact. The gut microbiota has a major role in shaping honey bees tolerance and resistance to parasite infestation and viral infection, but the contribution of viruses to the assembly of the host microbiota in the context of varroa resistance and susceptibility remains unclear. Here, we used a network approach including viral and bacterial nodes to characterize the impact of five viruses, Apis Rhabdovirus-1 (ARV-1), Black Queen Cell virus (BQCV), Lake Sinai virus (LSV), Sacbrood virus (SBV) and Deformed wing virus (DWV) on the gut microbiota assembly of varroa-susceptible and Gotland varroa-surviving honey bees. We found that microbiota assembly was different in varroa-surviving and varroa-susceptible honey bees with the network of the latter having a whole module not present in the network of the former. Four viruses, ARV-1, BQCV, LSV, and SBV, were tightly associated with bacterial nodes of the core microbiota of varroa-susceptible honey bees, while only two viruses BQCV and LSV, appeared correlated with bacterial nodes in varroa-surviving honey bees. In silico removal of viral nodes caused major re-arrangement of microbial networks with changes in nodes centrality and significant reduction of the networks' robustness in varroa-susceptible, but not in varroa-surviving honey bees. Comparison of predicted functional pathways in bacterial communities using PICRUSt2 showed the superpathway for heme b biosynthesis from uroporphyrinogen-III and a pathway for arginine, proline, and ornithine interconversion as significantly increased in varroa-surviving honey bees. Notably, heme and its reduction products biliverdin and bilirubin have been reported as antiviral agents. These findings show that viral pathogens are differentially nested in the bacterial communities of varroa-surviving and varroa-susceptible honey bees. These results suggest that Gotland honey bees are associated with minimally-assembled and reduced bacterial communities that exclude viral pathogens and are resilient to viral nodes removal, which, together with the production of antiviral compounds, may explain the resiliency of Gotland honey bees to viral infections. In contrast, the intertwined virus-bacterium interactions in varroa-susceptible networks suggest that the complex assembly of microbial communities in this honey bee strain favor viral infections, which may explain viral persistence in this honey bee strain. Further understanding of protective mechanisms mediated by the microbiota could help developing novel ways to control devastating viral infections affecting honey bees worldwide. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2024 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0944501323001209/pdfft?md5=5e3a9007d61be92b35088f031b11fe4b&pid=1-s2.0-S0944501323001209-main.pdf
Počet záznamů: 1