Počet záznamů: 1
Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle
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SYSNO ASEP 0570490 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle Tvůrce(i) Janovská, Petra (FGU-C) RID, ORCID
Zouhar, Petr (FGU-C) RID, ORCID, SAI
Bardová, Kristina (FGU-C) RID, ORCID, SAI
Otáhal, Jakub (FGU-C) RID, ORCID, SAI
Vrbacký, Marek (FGU-C) RID, ORCID
Mráček, Tomáš (FGU-C) RID, ORCID
Adamcová, Kateřina (FGU-C) ORCID, RID
Leňková, Lucie (FGU-C) ORCID, RID
Funda, Jiří (FGU-C) ORCID
Čajka, Tomáš (FGU-C) RID, ORCID, SAI
Drahota, Zdeněk (FGU-C) RID, ORCID
Stanić, Sara (FGU-C)
Rustan, A. C. (NO)
Horáková, Olga (FGU-C) RID, ORCID
Houštěk, Josef (FGU-C) RID, ORCID
Rossmeisl, Martin (FGU-C) RID, ORCID
Kopecký, Jan (FGU-C) RID, ORCIDČíslo článku 101683 Zdroj.dok. Molecular Metabolism. - : Elsevier - ISSN 2212-8778
Roč. 69, March (2023)Poč.str. 19 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova non-shivering thermogenesis ; sarcolipin ; mitochondrial supercomplex ; skeletal muscle ; brown adipose tissue ; obesity Obor OECD Endocrinology and metabolism (including diabetes, hormones) CEP LX22NPO5104 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-04483S GA ČR - Grantová agentura ČR GA19-02411S GA ČR - Grantová agentura ČR GA21-18993S GA ČR - Grantová agentura ČR GA22-07004S GA ČR - Grantová agentura ČR Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000964009500001 EID SCOPUS 85149068713 DOI 10.1016/j.molmet.2023.101683 Anotace Objective:Non-shivering thermogenesis (NST) mediated by uncoupling protein 1 (UCP1) in brown adipose tissue (BAT) can be activated via the adrenergic system in response to cold or diet, contributing to both thermal and energy homeostasis. Other mechanisms, including metabolism of skeletal muscle, may also be involved in NST. However, relative contribution of these energy dissipating pathways and their adaptability remain a matter of long-standing controversy.Methods:We used warm-acclimated (30 °C) mice to characterize the effect of an up to 7-day cold acclimation (6 °C, CA) on thermoregulatory thermogenesis, comparing inbred mice with a genetic background conferring resistance (A/J) or susceptibility (C57BL/6 J) to obesity.Results:Both warm-acclimated C57BL/6 J and A/J mice exhibited similar cold endurance, assessed as a capability to maintain core body temperature during acute exposure to cold, which improved in response to CA, resulting in comparable cold endurance and similar induction of UCP1 protein in BAT of mice of both genotypes. Despite this, adrenergic NST in BAT was induced only in C57BL/6 J, not in A/J mice subjected to CA. Cold tolerance phenotype of A/J mice subjected to CA was not based on increased shivering, improved insulation, or changes in physical activity. On the contrary, lipidomic, proteomic and gene expression analyses along with palmitoyl carnitine oxidation and cytochrome c oxidase activity revealed induction of lipid oxidation exclusively in skeletal muscle of A/J mice subjected to CA. These changes appear to be related to skeletal muscle NST, mediated by sarcolipin-induced uncoupling of sarco(endo)plasmic reticulum calcium ATPase pump activity and accentuated by changes in mitochondrial respiratory chain supercomplexes assembly.Conclusions:Our results suggest that NST in skeletal muscle could be adaptively augmented in the face of insufficient adrenergic NST in BAT, depending on the genetic background of the mice. It may provide both protection from cold and resistance to obesity, more effectively than BAT. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.molmet.2023.101683
Počet záznamů: 1