Počet záznamů: 1
Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans
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SYSNO ASEP 0569079 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparison of molecular diagnostic approaches for the detection and differentiation of the intestinal protist Blastocystis sp. in humans Tvůrce(i) Šloufová, Martina (BC-A) RID
Lhotská, Zuzana (BC-A) RID, ORCID
Jirků, Milan (BC-A) RID
Petrželková, Klára Judita (BC-A) RID
Stensvold, C.R. (DK)
Cinek, O. (CZ)
Pomajbíková, Kateřina (BC-A) RID, ORCIDCelkový počet autorů 7 Číslo článku 30 Zdroj.dok. Parasite - ISSN 1252-607X
Roč. 29, MAY (2022)Poč.str. 7 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova Blastocystis ; Conventional-PCR ; qPCR ; Sensitivity ; Quantification ; ngs Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Parasitology CEP GA22-04837S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000803306000001 EID SCOPUS 85131203128 DOI 10.1051/parasite/2022029 Anotace Blastocystis is the most commonly found intestinal protist in the world. Accurate detection and differentiation of Blastocystis including its subtypes (arguably species) are essential to understand its epidemiology and role in human health. We compared (i) the sensitivity of conventional PCR (cPCR) and qPCR in a set of 288 DNA samples obtained from stool samples of gut-healthy individuals, and (ii) subtype diversity as detected by next-generation sequencing (NGS) versus Sanger sequencing. Real-time PCR resulted in more positive samples than cPCR, revealing high fecal load of Blastocystis based on the quantification curve in most samples. In subtype detection, NGS was largely in agreement with Sanger sequencing but showed higher sensitivity for mixed subtype colonization within one host. This fact together with use of the combination of qPCR and NGS and obtaining information on the fecal protist load will be beneficial for epidemiological and surveillance studies. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220019/parasite220019.html
Počet záznamů: 1