Počet záznamů: 1
Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
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SYSNO ASEP 0565107 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice Tvůrce(i) Mimouni, N. E. H. (FR)
Ialy-Radio, C. (FR)
Denizot, A. (FR)
Lagoutte, I. (FR)
Frolíková, Michaela (BTO-N) ORCID
Komrsková, Kateřina (BTO-N) ORCID
Barbaux, S. (FR)
Ziyyat, A. (FR)Celkový počet autorů 8 Číslo článku 13812 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 22 (2022)Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova integrin beta1 ; Stra8-Cre ; sperm ; fertilization ; ultrasound Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA22-30494S GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000887554300001 EID SCOPUS 85142827946 DOI 10.3390/ijms232213812 Anotace Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm-oocyte adhesion/fusion and early implantation in the absence of sperm beta 1 integrin. Males and females with beta 1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm beta 1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin beta 1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2023 Elektronická adresa https://www.mdpi.com/1422-0067/23/22/13812
Počet záznamů: 1