Počet záznamů: 1
Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae)
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SYSNO ASEP 0564481 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název Good practices for histological analysis of the annual killifish Nothobranchius furzeri (Nothobranchiidae) Tvůrce(i) Žák, Jakub (UBO-W) RID, ORCID, SAI
Dyková, I. (CZ)
Blažek, Radim (UBO-W) RID, ORCID, SAI
Reichard, Martin (UBO-W) RID, ORCID, SAI, SAICelkový počet autorů 4 Zdroj.dok. Cold Spring Harbor protocols. - : Cold Spring Harbor Laboratory Press - ISSN 1940-3402
Roč. 2022, č. 11 (2022), s. 574-583Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Animals ; Eosine Yellowish-(YS) ; Fundulidae ; Microtomy ; Paraffin ; Staining and Labeling Vědní obor RIV EG - Zoologie Obor OECD Zoology Způsob publikování Omezený přístup Institucionální podpora UBO-W - RVO:68081766 EID SCOPUS 85141888652 DOI 10.1101/pdb.prot107739 Anotace Paraffin histology is one of the most important and commonly used laboratory techniques enabling the study of the microscopic structure of animal and plant tissues. This technique uses paraffin wax, which in liquid form impregnates fixed and dehydrated tissues and allows the preparation of thin sections when solidified in blocks. This protocol on good practices in paraffin histology of Nothobranchius furzeri (Nothobranchiidae) summarizes the authors' current experience in terms of technique, evaluation, and interpretation of sectioned tissues. The steps that precede paraffin block preparation are also presented as they play a key role in maximizing the quality of examined sections. The paraffin technique as described only requires basic laboratory conditions to produce good-quality results. The description of staining methods is limited to Mayer's hematoxylin and eosin (H&E), the routinely used histological dye staining cell nuclei in blue-black (hematein) and cell cytoplasm and connective tissue fibers in shades of pink-red (eosin). Killifish specialists are encouraged to engage in the study of histology and histopathology, taking advantage of interdisciplinary cooperation. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2023 Elektronická adresa http://cshprotocols.cshlp.org/content/2022/11/pdb.prot107739.long
Počet záznamů: 1