Toll-Like Receptor 3 Overexpression Induces Invasion of Prostate Cancer Cells, whereas Its Activation Triggers Apoptosis

Muresan, Ximena Maria; Slabáková, Eva; Procházková, Jiřina; Drápela, Stanislav; Fedr, Radek; Pícková, Markéta; Vacek, Antonín; Víchová, Ráchel; Suchánková, Tereza; Bouchal, J.; Kurfurstova, D.; Král, M.; Hulinova, T.; Sykora, R.; Študent, V.; Hejret, V.; van Weerden, W.; Puhr, M.; Pustka, V.; Potěšil, D.; Zdráhal, Z.; Culig, Z.; Souček, Karel

doi:https://dx.doi.org/10.1016/j.ajpath.2022.05.009

Počet záznamů: 1

Toll-Like Receptor 3 Overexpression Induces Invasion of Prostate Cancer Cells, whereas Its Activation Triggers Apoptosis

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SYSNO ASEP	0562613
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Toll-Like Receptor 3 Overexpression Induces Invasion of Prostate Cancer Cells, whereas Its Activation Triggers Apoptosis
Tvůrce(i)	Muresan, Ximena Maria (BFU-R)_ORCID Slabáková, Eva (BFU-R)_{RID, ORCID} Procházková, Jiřina (BFU-R)_{RID, ORCID} Drápela, Stanislav (BFU-R)_ORCID Fedr, Radek (BFU-R)_ORCID Pícková, Markéta (BFU-R)_ORCID Vacek, Antonín (BFU-R) Víchová, Ráchel (BFU-R) Suchánková, Tereza (BFU-R)_{RID, ORCID} Bouchal, J. (CZ) Kurfurstova, D. (CZ) Král, M. (CZ) Hulinova, T. (CZ) Sykora, R. (CZ) Študent, V. (CZ) Hejret, V. (CZ) van Weerden, W. (NL) Puhr, M. (AT) Pustka, V. (CZ) Potěšil, D. (CZ) Zdráhal, Z. (CZ) Culig, Z. (CZ) Souček, Karel (BFU-R)_{RID, ORCID}
Celkový počet autorů	23
Zdroj.dok.	American Journal of Pathology. - : Elsevier - ISSN 0002-9440 Roč. 192, č. 9 (2022), s. 1321-1335
Poč.str.	15 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	DOCETAXEL RESISTANCE ; EPITHELIAL-CELLS ; TUMOR-GROWTH
Vědní obor RIV	EC - Imunologie
Obor OECD	Pathology
Způsob publikování	Omezený přístup
Institucionální podpora	BFU-R - RVO:68081707
UT WOS	000859598300009
EID SCOPUS	85137156103
DOI	10.1016/j.ajpath.2022.05.009
Anotace	Toll-like receptor 3 (TLR3) is an endosomal receptor expressed in several immune and epithelial cells. Recent studies have highlighted its expression also in solid tumors, including prostate cancer (PCa), and have described its role primarily in the proinflammatory response and induction of apoptosis. It is up-regulated in some castration-resistant prostate cancers. However, the role of TLR3 in prostate cancer progression remains largely unknown. The current study experimentally demonstrated that exogenous TLR3 activation in PCa cell lines leads to a significant induction of secretion of the cytokines IL-6, IL-8, and interferonb, depending on the model and chemoresistance status. Transcriptomic analysis of TLR3-overexpressing cells revealed a functional program that is enriched for genes involved in the regulation of cell motility, migration, and tumor invasiveness. Increased motility, migration, and invasion in TLR3-overexpressing cell line were confirmed by several in vitro assays and using an orthotopic prostate xenograft model in vivo. Furthermore, TLR3-ligand induced apoptosis via cleavage of caspase-3/7 and poly (ADP-ribose) polymerase, predominantly in TLR3-overexpressing cells. These results indicate that TLR3 may be involved in prostate cancer progression and metastasis, however, it might also represent an Achilles heel of PCa, which can be exploited for targeted therapy. (Am J Pathol 2022, 192: 1321e1335, https://doi.org/10.1016/j.ajpath.2022.05.009)
Pracoviště	Biofyzikální ústav
Kontakt	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Rok sběru	2023
Elektronická adresa	https://doi.org/10.1016/j.ajpath.2022.05.009

Počet záznamů: 1