Počet záznamů: 1
Ionic Strength and Solution Composition Dictate the Adsorption of Cell-Penetrating Peptides onto Phosphatidylcholine Membranes
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SYSNO ASEP 0561693 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ionic Strength and Solution Composition Dictate the Adsorption of Cell-Penetrating Peptides onto Phosphatidylcholine Membranes Tvůrce(i) Nguyen, Man Thi Hong (UOCHB-X) ORCID
Biriukov, Denys (UOCHB-X) ORCID, RID
Tempra, Carmelo (UOCHB-X) ORCID
Baxová, Katarína (UOCHB-X)
Martinez-Seara, Hector (UOCHB-X) RID, ORCID
Evci, Huseyin (UFCH-W)
Singh, Vandana (UFCH-W)
Šachl, Radek (UFCH-W) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Jungwirth, Pavel (UOCHB-X) RID, ORCID
Javanainen, Matti (UOCHB-X) RID, ORCID
Vazdar, Mario (UOCHB-X) ORCIDZdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 38, č. 37 (2022), s. 11284-11295Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova linear constraint solver ; particle mesh Ewald ; molecular dynamics Obor OECD Physical chemistry CEP GX19-26854X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 ; UFCH-W - RVO:61388955 UT WOS 000855771400001 EID SCOPUS 85137926123 DOI 10.1021/acs.langmuir.2c01435 Anotace Adsorption of arginine-rich positively charged peptides onto neutral zwitterionic phosphocholine (PC) bilayers is a key step in the translocation of those potent cell-penetrating peptides into the cell interior. In the past, we have shown both theoretically and experimentally that polyarginines adsorb to the neutral PC-supported lipid bilayers in contrast to polylysines. However, comparing our results with previous studies showed that the results often do not match even at the qualitative level. The adsorption of arginine-rich peptides onto 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) may qualitatively depend on the actual experimental conditions where binding experiments have been performed. In this work, we systematically studied the adsorption of R9 and K9 peptides onto the POPC bilayer, aided by molecular dynamics (MD) simulations and fluorescence cross-correlation spectroscopy (FCCS) experiments. Using MD simulations, we tested a series of increasing peptide concentrations, in parallel with increasing Na+ and Ca2+ salt concentrations, showing that the apparent strength of adsorption of R9 decreases upon the increase of peptide or salt concentration in the system. The key result from the simulations is that the salt concentrations used experimentally can alter the picture of peptide adsorption qualitatively. Using FCCS experiments with fluorescently labeled R9 and K9, we first demonstrated that the binding of R9 to POPC is tighter by almost 2 orders of magnitude compared to that of K9. Finally, upon the addition of an excess of either Na+ or Ca2+ ions with R9, the total fluorescence correlation signal is lost, which implies the unbinding of R9 from the PC bilayer, in agreement with our predictions from MD simulations. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://doi.org/10.1021/acs.langmuir.2c01435
Počet záznamů: 1