Počet záznamů: 1
Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties
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SYSNO ASEP 0561666 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties Tvůrce(i) Ragaller, F. (SE)
Andronico, L. (SE)
Sýkora, Jan (UFCH-W) RID
Kulig, W. (FI)
Rog, T. (FI)
Urem, Y. B. (SE)
Abhinav, Abhinav (UFCH-W)
Danylchuk, D. I. (FR)
Hof, Martin (UFCH-W) RID, ORCID
Klymchenko, A. (FR)
Amaro, Mariana (UFCH-W) RID, ORCID
Vattulainen, I. (FI)
Sezgin, E. (SE)Číslo článku 220175 Zdroj.dok. Open Biology. - : Royal Society Publishing
Roč. 12, č. 9 (2022)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova environment-sensitive probes ; spectralimaging ; lipid saturation Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GX19-26854X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000874962300003 EID SCOPUS 85137790719 DOI 10.1098/rsob.220175 Anotace The plasma membrane, as a highly complex cell organelle, serves as a crucial platform for a multitude of cellular processes. Its collective biophysical properties are largely determined by the structural diversity of the different lipid species it accommodates. Therefore, a detailed investigation of biophysical properties of the plasma membrane is of utmost importance for a comprehensive understanding of biological processes occurring therein. During the past two decades, several environment-sensitive probes have been developed and become popular tools to investigate membrane properties. Although these probes are assumed to report on membrane order in similar ways, their individual mechanisms remain to be elucidated. In this study, using model membrane systems, we characterized the probes Pro12A, NR12S and NR12A in depth and examined their sensitivity to parameters with potential biological implications, such as the degree of lipid saturation, double bond position and configuration (cis versus trans), phospholipid headgroup and cholesterol content. Applying spectral imaging together with atomistic molecular dynamics simulations and time-dependent fluorescent shift analyses, we unravelled individual sensitivities of these probes to different biophysical properties, their distinct localizations and specific relaxation processes in membranes. Overall, Pro12A, NR12S and NR12A serve together as a toolbox with a wide range of applications allowing to select the most appropriate probe for each specific research question. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2023 Elektronická adresa https://hdl.handle.net/11104/0334190
Počet záznamů: 1