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Monitoring cells local temperature variation using nitrogen-vacancy (NV) centers in nanodiamonds
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SYSNO ASEP 0560715 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Monitoring cells local temperature variation using nitrogen-vacancy (NV) centers in nanodiamonds Tvůrce(i) Petrini, G. (IT)
Tomagra, G. (IT)
Bernardi, E. (IT)
Moreva, E. (IT)
Traina, P. (IT)
Marcantoni, A. (IT)
Picollo, F. (IT)
Olivero, P. (IT)
Kvaková, Klaudia (UOCHB-X) ORCID
Cígler, Petr (UOCHB-X) RID, ORCID
Degiovanni, I. P. (IT)
Carabelli, V. (IT)
Genovese, M. (IT)Zdroj.dok. 2022 IEEE International Workshop on Metrology for Industry 4.0 and IoT, MetroInd 4.0 and IoT. - Piscataway : IEEE, 2022 - ISBN 978-166541093-9 Rozsah stran s. 121-125 Poč.str. 5 s. Forma vydání Tištěná - P Akce IEEE International Workshop on Metrology for Industry 4.0 and IoT, MetroInd 4.0 and IoT 2022 Datum konání 07.06.2022 - 09.06.2022 Místo konání Trento Země IT - Itálie Typ akce EUR Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova biological applications ; cell temperature ; neurons ; NV center ; quantum sensors Obor OECD Nano-materials (production and properties) Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000855570300023 EID SCOPUS 85136153535 DOI 10.1109/MetroInd4.0IoT54413.2022.9831763 Anotace The growing interest in understanding the complex mechanisms that regulate biological processes has prompted the study and the improvement of quantum sensors, potentially capable of detecting a wide class of physical quantities of biological interest. Among the various sensors proposed for biological applications, nitrogen-vacancy (NV) centers in artificial diamond have emerged as a truly promising solution primarily thanks to their excellent bio-compatibility. Such NV sensors can be synthesized of nanometer size. The nanodiamonds can be inserted inside cells and, if properly functionalized, they can be targeted to organelles, such as mitochondria, or ion channels. In addition to the advantages regarding their chemical and structural composition, the NV sensors have distinguished themselves thanks to their sensitivity respect different physical quantity, such as magnetic and electric fields, temperatures and pressures variations. Although the sensitivity achieved by the NV quantum sensors is not yet sufficient to detect the very weak electromagnetic fields generated by biological processes, the thermal variation generated at the cellular level seem at the moment a more attractive field of application. Temperature is an important parameter for the regulation of intracellular processes and its detection is fundamental for a more complete understanding of them. Cellular activity and metabolism can affect the local temperature in cells and pathological conditions such as cancer, Parkinson and Alzherimer's disease can alter it. In this sense, local temperature monitoring within cells is also important for clinical application. Here we will present our experimental setup dedicated to local temperature measurement in neuronal cell cultures. The measurement technique is based on optically detected magnetic resonance (ODMR) with the NV centers in the nanodiamonds, suitably engineered to be sensitive and at the same time bio-compatible. We will demonstrate a proof of principle experiment in which we measure the local temperature variation in cultured hippocampal neurons. The temperature sensitivity is 3 K/Hz l/2 . In addition we will show how the nanodiamonds with a size of around 200 nm are internalized by the neurons. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://ieeexplore.ieee.org/abstract/document/9831763
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