Počet záznamů: 1
Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology
- 1.
SYSNO ASEP 0555493 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology Tvůrce(i) Soukal, P. (CZ)
Hrdá, Š. (CZ)
Karnkowska, A. (PL)
Milanowski, R. (PL)
Szabová, J. (CZ)
Hradilová, Miluše (UMG-J)
Strnad, Hynek (UMG-J) RID
Vlček, Čestmír (UMG-J) RID
Čepička, I. (CZ)
Hampl, V. (CZ)Celkový počet autorů 10 Číslo článku 13070 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Poč.str. 17 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova phylogenetic analysis ; energy-metabolism ; sequence ; introns ; genome ; rna ; alignment ; proteins ; gracilis ; identification Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000667449400007 DOI 10.1038/s41598-021-92174-3 Anotace Euglenids represent a group of protists with diverse modes of feeding. To date, only a partial genomic sequence of Euglena gracilis and transcriptomes of several phototrophic and secondarily osmotrophic species are available, while primarily heterotrophic euglenids are seriously undersampled. In this work, we begin to fill this gap by presenting genomic and transcriptomic drafts of a primary osmotroph, Rhabdomonas costata. The current genomic assembly length of 100 Mbp is 14x smaller than that of E. gracilis. Despite being too fragmented for comprehensive gene prediction it provided fragments of the mitochondrial genome and comparison of the transcriptomic and genomic data revealed features of its introns, including several candidates for nonconventional types. A set of 39,456 putative R. costata proteins was predicted from the transcriptome. Annotation of the mitochondrial core metabolism provides the first data on the facultatively anaerobic mitochondrion of R. costata, which in most respects resembles the mitochondrion of E. gracilis with a certain level of streamlining. R. costata can synthetise thiamine by enzymes of heterogenous provenances and haem by a mitochondrial-cytoplasmic C4 pathway with enzymes orthologous to those found in E. gracilis. The low percentage of green algae-affiliated genes supports the ancestrally osmotrophic status of this species. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s41598-021-92174-3
Počet záznamů: 1