Počet záznamů: 1
Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts
- 1.
SYSNO ASEP 0555470 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Desmoplastic Crosstalk in Pancreatic Ductal Adenocarcinoma Is Reflected by Different Responses of Panc-1, MIAPaCa-2, PaTu-8902, and CAPAN-2 Cell Lines to Cancer-associated/Normal Fibroblasts Tvůrce(i) Novák, S. (CZ)
Kolář, Michal (UMG-J) RID, ORCID
Szabo, A. (CZ)
Vernerová, Z. (CZ)
Lacina, L. (CZ)
Strnad, Hynek (UMG-J) RID
Šáchová, Jana (UMG-J)
Hradilová, Miluše (UMG-J)
Havránek, Jan (UMG-J)
Španko, M. (CZ)
Coma, M. (SK)
Urban, L. (SK)
Kaňuchová, M. (SK)
Melegova, N. (SK)
Gürlich, R. (CZ)
Dvořák, J. (CZ)
Smetana, K. (CZ)
Gal, P. (SK)
Szabo, P. (CZ)Celkový počet autorů 19 Zdroj.dok. Cancer Genomics & Proteomics. - : International Institute of Anticancer Research - ISSN 1109-6535
Roč. 18, č. 3 (2021), s. 221-243Poč.str. 23 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GR - Řecko Klíč. slova Epithelial-mesenchymal interaction ; tumor micro environment ; cancer stem cell ; tumor stroma ; pancreas Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000643733600004 DOI 10.21873/cgp.20254 Anotace Background/Aim: Pancreatic ductal adenocarcinoma (PDAC) still represents one of the most aggressive cancers. Understanding of the epithelial-mesenchymal crosstalk as a crucial part of the tumor microenvironment should pave the way for therapies to improve patient survival rates. Well-established cell lines present a useful and reproducible model to study PDAC biology. However, the tumor-stromal interactions between cancer cells and cancer-associated fibroblasts (CAFs) are still poorly understood. Materials and Methods: We studied interactions between four PDAC cell lines (Panc-1, CAPAN-2, MIAPaCa-2, and PaTu-8902) and conditioned media derived from primary cultures of normal fibroblasts/PDAC-derived CAFs (PANFs). Results: When the tested PDAC cell lines were stimulated by PANF-derived conditioned media, the most aggressive behavior was acquired by the Panc-1 cell line (increased number and size of colonies, remaining expression of vimentin and keratin 8 as well as increase of epithelial-to-mesenchymal polarization markers), whereas PaTu-8902 cells were rather inhibited. Of note, administration of the conditioned media to MIAPaCa-2 cells resulted in an inverse effect on the size and number of colonies, whereas CAPAN-2 cells were rather stimulated. To explain the heterogeneous pattern of the observed PDAC crosstalk at the in vitro level, we further compared the phenotype of primary cultures of cells derived from ascitic fluid with that of the tested PDAC cell lines, analyzed tumor samples of PDAC patients, and performed gene expression profiling of PANFs. Immuno-cyto/histo-chemical analysis found specific phenotype differences within the group of examined patients and tested PDAC cell lines, whereas the genomic approach in PANFs found the key molecules (IL6, IL8, MFGE8 and periostin) that may contribute to the cancer aggressive behavior. Conclusion: The desmoplastic patient-specific regulation of cancer cells by CAFs (also demonstrated by the heterogeneous response of PDAC cell lines to fibroblasts) precludes simple targeting and development of an effective treatment strategy and rather requires establishment of an individualized tumor-specific treatment protocol. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2022 Elektronická adresa https://cgp.iiarjournals.org/content/18/3/221
Počet záznamů: 1