Počet záznamů: 1
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses
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SYSNO ASEP 0554898 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses Tvůrce(i) Tidu, F. (CZ)
De Zuani, M. (CZ)
Jose, S. (CZ)
Bendíčková, K. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Caruso, F. (AU)
Cavalieri, F. (AU)
Forte, G. (CZ)
Frič, J. (CZ)Celkový počet autorů 9 Číslo článku 102683 Zdroj.dok. iScience. - : Cell Press
Roč. 24, č. 6 (2021)Poč.str. 25 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova toll-like receptors ; beta-glucan recognition ; c-type lectin ; stem-cells ; hyaluronan degradation ; activation Obor OECD Biophysics Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000667301700118 EID SCOPUS 85109156613 DOI 10.1016/j.isci.2021.102683 Anotace Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties of MSCs seem beneficial, however, their response during fungal infection, which is an important cause of mortality in patients with GvHD, is unknown. We report that MSCs phagocytose the fungal component zymosan, resulting in phosphorylation of spleen tyrosine kinase (Syk), increase in cytosolic calcium levels, and ultimately, increase in NFAT1 nuclear translocation. RNA sequencing analysis of zymosan-treated MSCs showed that CN-NFAT inhibition affects extracellular matrix (ECM) genes but not cytokine expression that is under the control of the NF-kappa B pathway. When coculturing MSCs or decellularized MSC-ECM with human peripheral blood mononuclear cells (PBMCs), selective NFAT inhibition in MSCs decreased cytokine expression by PBMCs. These findings reveal a dual mechanism underlying the MSC response to zymosan: while NF-kappa B directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2589004221006519?via%3Dihub#!
Počet záznamů: 1