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Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs
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SYSNO ASEP 0553281 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs Tvůrce(i) Zhu, H. (CN)
Hefka Blahnová, Veronika (UEM-P)
Perale, G. (AT)
Xiao, J. (CN)
Betge, F. (CZ)
Boniolo, F. (DE)
Filová, Eva (UEM-P) RID, ORCID
Lyngstadaas, S. P. (NO)
Haugen, A. (NO)Číslo článku 619111 Zdroj.dok. Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 8, dec. (2020)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova bone scaffold ; bone graft ; bone regeneration biomimetic ; bioactive proteins ; intrinsically disordered ; mesenchymal stem cells ; xenograft Obor OECD Biomaterials (as related to medical implants, devices, sensors) Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000604734100001 EID SCOPUS 85099013632 DOI 10.3389/fcell.2020.619111 Anotace Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep(R), with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep(R) has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fcell.2020.619111/full
Počet záznamů: 1