Počet záznamů: 1
Reversible Lectin Binding to Glycan-Functionalized Graphene
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SYSNO ASEP 0551978 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Reversible Lectin Binding to Glycan-Functionalized Graphene Tvůrce(i) Koukalová, Tereza (MBU-M)
Kovaříček, P. (CZ)
Bojarová, Pavla (MBU-M) ORCID
Guerra, V. L. P. (CZ)
Vrkoslav, V. (CZ)
Navara, L. (CZ)
Jirka, I. (CZ)
Cebecauer, M. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Kalbáč, M. (CZ)Celkový počet autorů 10 Číslo článku 6661 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 13 (2021)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova copper ; graphene ; wheat germ agglutinin ; carbohydrate ; 2D materials ; sensor Vědní obor RIV JJ - Ostatní materiály Obor OECD Nano-materials (production and properties) CEP LTC20072 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000671186500001 EID SCOPUS 85108207108 DOI 10.3390/ijms22136661 Anotace The monolayer character of two-dimensional materials predestines them for application as active layers of sensors. However, their inherent high sensitivity is always accompanied by a low selectivity. Chemical functionalization of two-dimensional materials has emerged as a promising way to overcome the selectivity issues. Here, we demonstrate efficient graphene functionalization with carbohydrate ligands-chitooligomers, which bind proteins of the lectin family with high selectivity. Successful grafting of a chitooligomer library was thoroughly characterized, and glycan binding to wheat germ agglutinin was studied by a series of methods. The results demonstrate that the protein quaternary structure remains intact after binding to the functionalized graphene, and that the lectin can be liberated from the surface by the addition of a binding competitor. The chemoenzymatic assay with a horseradish peroxidase conjugate also confirmed the intact catalytic properties of the enzyme. The present approach thus paves the way towards graphene-based sensors for carbohydrate-lectin binding. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/13/6661
Počet záznamů: 1