Počet záznamů: 1
The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
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SYSNO ASEP 0551826 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders Tvůrce(i) Mili, M. (HR)
Ceppi, M. (IT)
Bruzzone, M. (IT)
Azqueta, A. (ES)
Brunborg, G. (NO)
Godschalk, R. (NL)
Koppen, G. (BE)
Langie, S. (NL)
Moller, P. (DK)
Teixeira, J. P. (PT)
Alija, A. (XK)
Anderson, D. (GB)
Andrade, V. (HR)
Andreoli, C. (IT)
Asllani, F. (XK)
Boutet-Robinet, E. (NO)
Buschini, A. (IT)
Cavallo, D. (IT)
Pereira, C.C. (PT)
Costa, C. (PT)
Costa, S. (PT)
Da Silva, J. (BR)
Del Bo', C. (IT)
Sreckovic, V.D. (RS)
Djelic, N. (RS)
Duračková, Z. (PL)
Dvořáková, M. (SK)
Gajski, G. (HR)
Galati, S. (IT)
Lima, O.G. (CU)
Giovannelli, L. (IT)
Goroshinskaya, I.A. (RU)
Grindel, A. (AT)
Gützkow, K.B. (NO)
Hernandez, A. (ES)
Hernandez, C. (CU)
Holven, K.B. (NO)
Ibero-Baraibar, I. (ES)
Ottestad, I. (NO)
Kadioglu, E. (TR)
Kažimírová, A. (SK)
Kuznetsova, E. (RU)
Ladeira, C. (ES)
Laffon, B. (ES)
Lamonaca, P. (IT)
Lebailly, P. (FR)
Louro, H. (PT)
Cardoso, T.M. (CU)
Marcon, F. (IT)
Marcos, R. (ES)
Moretti, M. (IT)
Moretti, S. (IT)
Najafzadeh, M. (GB)
Németh, Z. (HU)
Neri, M. (IT)
Novotná, Božena (UEM-P)
Orlow, I. (FR)
Paduchova, Z. (SK)
Pastor, S. (ES)
Perdry, H. (FR)
Spremo-Potparevic, B. (RS)
Ramadhani, D. (ID)
Riso, P. (IT)
Rohr, P. (BR)
Rojas, E. (MX)
Rössner ml., Pavel (UEM-P) RID, ORCID
Safar, A. (HU)
Sardas, S. (TR)
Silva, M.J. (PT)
Sirota, N. (RU)
Smolková, B. (SK)
Staruchova, M. (TR)
Štětina, R. (CZ)
Stopper, H. (DE)
Surikova, E.I. (RU)
Ulven, S.M. (NO)
Ursini, C.L. (IT)
Valdiglesias, V. (ES)
Valverde, M. (MX)
Vodička, Pavel (UEM-P) RID
Volkovová, K. (SK)
Wagner, K.H. (AT)
Zivkovic, L. (RS)
Dusinska, M. (NO)
Collins, A. R. (NO)
Bonassi, S. (IT)Číslo článku 108371 Zdroj.dok. Mutation Research-Reviews in Mutation Research. - : Elsevier - ISSN 1383-5742
Roč. 787, jan-jun (2021)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova comet assay ; DNA damage ; pooled analysis ; human biomonitoring ; biomarkers Vědní obor RIV DN - Vliv životního prostředí na zdraví Obor OECD Genetics and heredity (medical genetics to be 3) CEP EF16_019/0000798 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000658539600011 EID SCOPUS 85101040695 DOI 10.1016/j.mrrev.2021.108371 Anotace The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project a COST Action launched in 2016 represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations. Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1383574221000089?via%3Dihub
Počet záznamů: 1