Počet záznamů: 1
Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats
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SYSNO ASEP 0550900 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats Tvůrce(i) Forostyak, Serhiy (UEM-P) RID, ORCID
Forostyak, Oksana (UEM-P)
Kwok, Jessica (UEM-P) ORCID, RID
Romanyuk, Nataliya (UEM-P) RID
Řehořová, Monika (UEM-P)
Kriška, Ján (UEM-P) RID, ORCID
Dayanithi, Govindan (UEM-P) RID
Raha-Chowdhury, R. (GB)
Jendelová, Pavla (UEM-P) RID, ORCID
Anděrová, Miroslava (UEM-P) RID, ORCID
Fawcett, James (UEM-P) ORCID
Syková, Eva (UEM-P) RIDČíslo článku 9593 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 24 (2020)Poč.str. 25 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova proteoglycans ; plasticity ; neurodegeneration ; stem cells ; iPS ; ALS Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA19-02046S GA ČR - Grantová agentura ČR EF15_003/0000419 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEM-P - RVO:68378041 UT WOS 000603507100001 EID SCOPUS 85098229897 DOI 10.3390/ijms21249593 Anotace A promising therapeutic strategy for amyotrophic lateral sclerosis (ALS) treatment is stem cell therapy. Neural progenitors derived from induced pluripotent cells (NP-iPS) might rescue or replace dying motoneurons (MNs). However, the mechanisms responsible for the beneficial effect are not fully understood. The aim here was to investigate the mechanism by studying the effect of intraspinally injected NP-iPS into asymptomatic and early symptomatic superoxide dismutase (SOD)1(G93A) transgenic rats. Prior to transplantation, NP-iPS were characterized in vitro for their ability to differentiate into a neuronal phenotype. Motor functions were tested in all animals, and the tissue was analyzed by immunohistochemistry, qPCR, and Western blot. NP-iPS transplantation significantly preserved MNs, slowed disease progression, and extended the survival of all treated animals. The dysregulation of spinal chondroitin sulfate proteoglycans was observed in SOD1(G93A) rats at the terminal stage. NP-iPS application led to normalized host genes expression (versican, has-1, tenascin-R, ngf, igf-1, bdnf, bax, bcl-2, and casp-3) and the protection of perineuronal nets around the preserved MNs. In the host spinal cord, transplanted cells remained as progenitors, many in contact with MNs, but they did not differentiate. The findings suggest that NP-iPS demonstrate neuroprotective properties by regulating local gene expression and regulate plasticity by modulating the central nervous system (CNS) extracellular matrix such as perineuronal nets (PNNs). Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/21/24/9593
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