Počet záznamů: 1
Quantifying Cyanothece growth under DIC limitation
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SYSNO ASEP 0550181 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Quantifying Cyanothece growth under DIC limitation Tvůrce(i) Inomura, K. (US)
Masuda, Takako (MBU-M) ORCID
Eichner, Meri (MBU-M) ORCID, RID
Rabouille, S. (FR)
Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Červený, Jan (UEK-B) RID, ORCID, SAI
Vancová, Marie (BC-A) RID, ORCID
Bernát, Gábor (MBU-M) ORCID
Armin, G. (US)
Claquin, P. (FR)
Kotabová, Eva (MBU-M) RID, ORCID
Stephan, S. (DE)
Suggett, D.J. (AU)
Deutsch, C. (US)
Prášil, Ondřej (MBU-M) RID, ORCIDZdroj.dok. Computational and Structural Biotechnology Journal. - : Elsevier - ISSN 2001-0370
Roč. 19, č. 2021 (2021), s. 6456-6464Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Cyanothece ; DIC ; CO2 ; Nitrogen fixation ; Nitrate ; Diurnal Cyanothece ; Carbon ; Photosynthesis ; Quantitative model Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Morfologické obory a cytologie
Ústav výzkumu globální změny - Mikrobiologie, virologieCEP GA20-17627S GA ČR - Grantová agentura ČR GA18-24397S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UEK-B - RVO:86652079 ; BC-A - RVO:60077344 UT WOS 000731411300006 EID SCOPUS 85120717280 DOI 10.1016/j.csbj.2021.11.036 Anotace The photoautotrophic, unicellular N-2-fixer, Cyanothece, is a model organism that has been widely used to study photosynthesis regulation, the structure of photosystems, and the temporal segregation of carbon (C) and nitrogen (N) fixation in light and dark phases of the diel cycle. Here, we present a simple quantitative model and experimental data that together, suggest external dissolved inorganic carbon (DIC) concentration as a major limiting factor for Cyanothece growth, due to its high C-storage requirement. Using experimental data from a parallel laboratory study as a basis, we show that after the onset of the light period, DIC was rapidly consumed by photosynthesis, leading to a sharp drop in the rate of photosynthesis and C accumulation. In N-2-fixing cultures, high rates of photosynthesis in the morning enabled rapid conversion of DIC to intracellular C storage, hastening DIC consumption to levels that limited further uptake. The N-2-fixing condition allows only a small fraction of fixed C for cellular growth since a large fraction was reserved in storage to fuel night-time N-2 fixation. Our model provides a framework for resolving DIC limitation in aquatic ecosystem simulations, where DIC as a growth-limiting factor has rarely been considered, and importantly emphasizes the effect of intracellular C allocation on growth rate that varies depending on the growth environment. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S2001037021005018?via%3Dihub
Počet záznamů: 1