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Vaccinia Virus Expressing Interferon Regulatory Factor 3 Induces Higher Protective Immune Responses against Lethal Poxvirus Challenge in Atopic Organism
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SYSNO ASEP 0549782 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Vaccinia Virus Expressing Interferon Regulatory Factor 3 Induces Higher Protective Immune Responses against Lethal Poxvirus Challenge in Atopic Organism Tvůrce(i) Pilná, H. (CZ)
Hájková, V. (CZ)
Knitlová, Jarmila (FGU-C) RID, ORCID
Lišková, Jana (FGU-C) RID, ORCID
Elsterová, Jana (BC-A) ORCID
Melková, Z. (CZ)Číslo článku 1986 Zdroj.dok. Viruses. - : MDPI
Roč. 13, č. 10 (2021)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova IRF-3 ; vaccinia virus ; smallpox ; atopic dermatitis ; eczema vaccinatum ; immunization ; interferon beta ; interleukin-1 beta ; cytokines ; Nc/Nga mice Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Mikrobiologie, virologie CEP LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy TN01000013 GA TA ČR - Technologická agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; BC-A - RVO:60077344 UT WOS 000713116900001 EID SCOPUS 85116464105 DOI 10.3390/v13101986 Anotace Vaccinia virus (VACV) is an enveloped DNA virus from the Orthopoxvirus family, various strains of which were used in the successful eradication campaign against smallpox. Both original and newer VACV-based replicating vaccines reveal a risk of serious complications in atopic individuals. VACV encodes various factors interfering with host immune responses at multiple levels. In atopic skin, the production of type I interferon is compromised, while VACV specifically inhibits the phosphorylation of the Interferon Regulatory Factor 3 (IRF-3) and expression of interferons. To overcome this block, we generated a recombinant VACV-expressing murine IRF-3 (WR-IRF3) and characterized its effects on virus growth, cytokine expression and apoptosis in tissue cultures and in spontaneously atopic Nc/Nga and control Balb/c mice. Further, we explored the induction of protective immune responses against a lethal dose of wild-type WR, the surrogate of smallpox. We demonstrate that the overexpression of IRF-3 by WR-IRF3 increases the expression of type I interferon, modulates the expression of several cytokines and induces superior protective immune responses against a lethal poxvirus challenge in both Nc/Nga and Balb/c mice. Additionally, the results may be informative for design of other virus-based vaccines or for therapy of different viral infections. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1999-4915/13/10/1986
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