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Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures
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SYSNO ASEP 0548997 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cytokinins are involved in drought tolerance of Pinus radiata plants originating from embryonal masses induced at high temperatures Tvůrce(i) Castander-Olarieta, A. (ES)
Moncaleán, P. (ES)
Pereira, C. (ES)
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Petřík, Ivan (UEB-Q) ORCID
Pavlović, Iva (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
Goicoa, T. (ES)
Ugarte, M. D. (ES)
Montalbán, I. A. (ES)Celkový počet autorů 11 Zdroj.dok. Tree Physiology - ISSN 0829-318X
Roč. 41, č. 6 (2021), s. 912-926Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova delayed effects ; photosynthesis ; phytohormones ; radiata pine ; somatic plants ; stress ; water potential Obor OECD Biochemistry and molecular biology CEP EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000671960000003 EID SCOPUS 85108123677 DOI 10.1093/treephys/tpaa055 Anotace Vegetative propagation through somatic embryogenesis is an effective method to produce elite varieties and can be applied as a tool to study the response of plants to different stresses. Several studies show that environmental changes during embryogenesis could determine future plant development. Moreover, we previously reported that physical and chemical conditions during somatic embryogenesis can determine the protein, hormone and metabolite profiles, as well as the micromorphological and ultrastructural organization of embryonal masses and somatic embryos. In this sense, phytohormones are key players throughout the somatic embryogenesis process as well as during numerous stress-adaptation responses. In this work, we first applied different higherature regimes (30 °C, 4 weeks, 40 °C, 4 days, 50 °C, 5 min) during induction of Pinus radiata D. Don somatic embryogenesis, together with control temperature (23 °C). Then, the somatic plants regenerated from initiated embryogenic cell lines and cultivated in greenhouse conditions were subjected to drought stress and control treatments to evaluate survival, growth and several physiological traits (relative water content, water potential, photosynthesis, stomatal conductance and transpiration). Based on those preliminary results, even more extreme higherature regimes were applied during induction (40 °C, 4 h, 50 °C, 30 min, 60 °C, 5 min) and the corresponding cytokinin profiles of initiated embryonal masses from different lines were analysed. The results showed that the temperature regime during induction had delayed negative effects on drought resilience of somatic plants as indicated by survival, photosynthetic activity and water- use efficiency. However, high temperatures for extended periods of time enhanced subsequent plant growth in well-watered conditions. Higherature regime treatments induced significant differences in the profile of total cytokinin bases, N6-isopentenyladenine, cis-zeatin riboside and trans-zeatin riboside. We concluded that phytohormones could be potential regulators of stress-response processes during initial steps of somatic embryogenesis and that they may have delayed implications in further developmental processes, determining the performance of the generated plants. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.1093/treephys/tpaa055
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