Počet záznamů: 1
eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast
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SYSNO ASEP 0547363 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast Tvůrce(i) Mohammad, Mahabub Pasha (MBU-M) RID
Smirnova, Anna (MBU-M)
Gunišová, Stanislava (MBU-M) RID
Valášek, Leoš Shivaya (MBU-M) RID, ORCIDZdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 49, č. 15 (2021), s. 8743-8756Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova eukaryotic translation initiation ; messenger-rna ; re-initiation ; promote ; gcn4 ; complex ; 4g ; binding ; domain ; perspective Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GX19-25821X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000697383500028 EID SCOPUS 85116537343 DOI 10.1093/nar/gkab652 Anotace Translation reinitiation is a gene-specific translational control mechanism. It is characterized by the ability of short upstream ORFs to prevent full ribosomal recycling and allow the post-termination 40S subunit to resume traversing downstream for the next initiation event. It is well known that variable transcript-specific features of various uORFs and their prospective interactions with initiation factors lend them an unequivocal regulatory potential. Here, we investigated the proposed role of the major initiation scaffold protein eIF4G in reinitiation and its prospective interactions with uORF's cis-acting features in yeast. In analogy to the eIF3 complex, we found that eIF4G and eIF4A but not eIF4E (all constituting the eIF4F complex) are preferentially retained on ribosomes elongating and terminating on reinitiation-permissive uORFs. The loss of the eIF4G contact with eIF4A specifically increased this retention and, as a result, increased the efficiency of reinitiation on downstream initiation codons. Combining the eIF4A-binding mutation with that affecting the integrity of the eIF4G1-RNA2-binding domain eliminated this specificity and produced epistatic interaction with a mutation in one specific cis-acting feature. We conclude that similar to humans, eIF4G is retained on ribosomes elongating uORFs to control reinitiation also in yeast. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://academic.oup.com/nar/article/49/15/8743/6342458
Počet záznamů: 1