Počet záznamů: 1
Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser
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SYSNO ASEP 0546207 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser Tvůrce(i) Juncheng, E. (DE)
Stransky, M. (DE)
Jurek, Z. (DE)
Fortmann-Grote, C. (DE)
Juha, Libor (FZU-D) RID, ORCID, SAI
Santra, R. (DE)
Ziaja, B. (PL)
Mancuso, A.P. (DE)Celkový počet autorů 8 Číslo článku 17976 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova X-ray Free-electron laser ; radiation damage ; inelastic scattering ; single-particle imaging of hydrated proteins Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) CEP LTT17015 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FZU-D - RVO:68378271 UT WOS 000695272000099 EID SCOPUS 85114771514 DOI 10.1038/s41598-021-97142-5 Anotace We present a computational case study of X-ray single-particle imaging of hydrated proteins on an example of 2-Nitrogenase–Iron protein covered with water layers of various thickness, using a start-to-end simulation platform and experimental parameters of the SPB/SFX instrument at the European X-ray Free-Electron Laser facility. The simulations identify an optimal thickness of the water layer at which the efective resolution for imaging the hydrated sample becomes signifcantly higher than for the non-hydrated sample. This efect is lost when the water layer becomes too thick. Even though the detailed results presented pertain to the specifc sample studied, the trends which we identify should also hold in a general case. We expect these fndings will guide future single-particle imaging experiments using hydrated proteins. Pracoviště Fyzikální ústav Kontakt Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Rok sběru 2022 Elektronická adresa http://hdl.handle.net/11104/0322754
Počet záznamů: 1