Počet záznamů: 1
Affiblot: Semi-quantitative Selection and Characterization of Research Antibodies
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SYSNO ASEP 0545733 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Affiblot: Semi-quantitative Selection and Characterization of Research Antibodies Tvůrce(i) Svobodová, Z. (CZ)
Novotný, Jakub (UIACH-O) ORCID
Ospálková, B. (CZ)
Nemčeková, K. (CZ)
Tripská, K. (CZ)
Foret, František (UIACH-O) RID, ORCID
Bílková, Z. (CZ)Celkový počet autorů 7 Zdroj.dok. MSB 2021 Final Program. -, 2021 Poč.str. 1 s. Forma vydání Online - E Akce International Symposium on Microscale Separations and Bioanalysis /37./ Datum konání 12.07.2021 - 15.07.2021 Místo konání Boston Země US - Spojené státy americké Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova binding reagents ; rubella ; validation Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Institucionální podpora UIACH-O - RVO:68081715 Anotace The affiblot is a dot blot-based palm-sized device that researchers, laboratories, and institutions can use to semi-quantitatively evaluate and select the most suitable antibody among different suppliers that fit their application in terms of affinity comparison, cross-reactivity, and batch-to-batch variability. The device is also able to control the condition of the antibodies for the duration of their storage. Moreover, this simple, fast, and flexible technique does not require special equipment, except a vacuum pump and camera. If more precise results are required, the ChemiDoc imaging system can be used instead of the camera. The target can be a purified or recombinant peptide, protein, or whole bacterial cell. This device could be used to compare any antibodies or affinity reagents that have not yet been on the market for a long time and are therefore not yet thoroughly validated, such as anti-SARS-CoV2 antibodies. These antibodies are currently widely used to reveal the mechanisms of virus replication and host-to-host transmission, the development of diagnostic methods, and clinical research. The dot blot-based device could help researchers rapidly select the most suitable and functional antibody for their application. Therefore, our future intent will include the evaluation of several anti-SARS-CoV-2 antibodies for research use. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2022 Elektronická adresa https://msb-conferences.org/detailed-program/
Počet záznamů: 1