Počet záznamů: 1
Affiblot: a dot blot-based screening device for selection of reliable antibodies
- 1.
SYSNO ASEP 0545112 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Affiblot: a dot blot-based screening device for selection of reliable antibodies Tvůrce(i) Svobodová, Z. (CZ)
Novotný, Jakub (UIACH-O) ORCID
Ospalková, B. (CZ)
Slováková, M. (CZ)
Bílková, Z. (CZ)
Foret, František (UIACH-O) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Analytical Methods: advancing methods and applications . - : Royal Society of Chemistry - ISSN 1759-9660
Roč. 13, č. 35 (2021), s. 3874-3884Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova binding reagents ; rubella ; validation Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000687267500001 EID SCOPUS 85115395353 DOI 10.1039/d1ay00955a Anotace The key factor in the development of antibody-based assays is to find an antibody that has an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not easy to carry out since the research antibodies on the market may suffer from low specificity and reproducibility. Here, we report on a palm-sized dot blot-based device, called the affiblot, that has a specially designed lid that allows simultaneous semi-quantitative comparison of up to five antibodies from different suppliers regarding their affinity/avidity, cross-reactivity, and batch-to-batch reliability. The only required peripheral equipment is a vacuum pump, a camera, and densitometry software. The affiblot device was tested for its functionality and its measurements were compared against those obtained by standard dot blot and ELISA. The benefit over these methods, when various antibodies are evaluated, is in its simplicity. It allows easy antigen deposition, fast application and the discarding of the solutions, a compact undivided membrane, and therefore significant decrease of labor. The device was tested with specific anti-ApoE, anti-EpCAM, anti-Salmonella, anti-E. coli, and anti-Listeria antibodies from different suppliers. Their properties were compared for their ability to interact specifically with antigen and/or non-target structures and the best-suited antibody for the intended application was identified. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2022 Elektronická adresa http://hdl.handle.net/11104/0321871
Počet záznamů: 1