Počet záznamů: 1
NAD-capping: a universal RNA modification in Mycobacteria, Archaea and Escherichia coli
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SYSNO ASEP 0544953 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název NAD-capping: a universal RNA modification in Mycobacteria, Archaea and Escherichia coli Tvůrce(i) Ruiz-Larrabeiti, Olatz (MBU-M) ORCID
Benoni, Roberto (UOCHB-X)
Zemlianski, V. (CZ)
Hanišáková, N. (CZ)
Suder, A. (PL)
Schwarz, Marek (MBU-M) ORCID, RID
Brezovská, Barbora (MBU-M)
Svojanovská, Barbora (UOCHB-X)
Vítězová, M. (CZ)
Cahová, Hana (UOCHB-X) RID, ORCID
Převorovský, M. (CZ)
Krásný, Libor (MBU-M) RID, ORCIDZdroj.dok. FEBS Open Bio . - : Wiley - ISSN 2211-5463
Roč. 11, Suppl 1 (2021), s. 18Poč.str. 1 s. Forma vydání Tištěná - P Akce FEBS Congress, Molecules of Life: Towards New Horizons /45./ Datum konání 03.07.2021 - 08.07.2021 Místo konání online Typ akce WRD Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Obor OECD Biochemistry and molecular biology Institucionální podpora UOCHB-X - RVO:61388963 ; MBU-M - RVO:61388971 UT WOS 000668898600046 Anotace The 50end status is key to the life of a transcript. NAD+(nicoti-namide adenine dinucleotide) universal redox cofactor has beendetected covalently attached to the 5’-end of RNA in bacterial and eukaryotic cells, where RNA polymerase uses NAD+insteadof ATP to initiate transcription. This new RNA modification hasbeen termed NAD-capping. Until now, NAD-capping had notbeen investigated in the mycobacterial genus, which containsimportant pathogens. At the same time, the nature of NAD+cofactor and the discovery of NAD-capping in a widespreadvariety of organisms raised up the possibility that NAD-cappingmay be a universal RNA modification, a hypothesis thatremained unproven until now. Likewise, a cohesive view of thebiological role of NAD-cap and its effect on the transcript andthe cell is still being formed. Here we show that NAD-cappingexists in mycobacteria, and that mycobacterial NAD-RNAs aremRNAs involved in membrane and redox processes and somesmall RNAs (sRNAs), like the characterized Ms1 and rnpB. Wehave also analysed archaeal RNA by mass spectrometry and dis-covered NAD+covalently attached to it, proofing that NAD-cap-ping is universal to all domains of life. Finally, we have studiedthe biological role of NAD-cap in RNAI, the most abundantlymodified sRNA in Escherichia coli. We found that preventingNAD-capping on RNAI caused it to have a longer half-life, andchanged the balance between RNAI and its regulatory target,which affected the ability of the cell to exit LAG phase at highampicillin concentrations, as compared to the wild-type. In thistalk I will explain how our discoveries on NAD-capping inMycobacteria, Archaea and Escherichia coli correlate to previousfindings, and how they add to our knowledge on transcriptomicmodifications. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2022
Počet záznamů: 1