Počet záznamů: 1
Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids
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SYSNO ASEP 0544779 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chromosome-wide characterization of meiotic noncrossovers (gene conversions) in mouse hybrids Tvůrce(i) Gergelits, Václav (UMG-J)
Parvanov, Emil (UMG-J)
Šimeček, Petr (UMG-J)
Forejt, Jiří (UMG-J) RID, ORCIDCelkový počet autorů 4 Číslo článku iyaa013 Zdroj.dok. Genetics. - : Genetics Society of America - ISSN 0016-6731
Roč. 217, č. 1 (2021)Poč.str. 14 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova homologous recombination ; gene conversion ; PRDM9 motif erosion ; hybrid sterility ; noncrossover-associated GC bias Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP LM2018126 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-04364S GA ČR - Grantová agentura ČR GA20-04075S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup UT WOS 000637217400010 DOI 10.1093/genetics/iyaa013 Anotace During meiosis, the recombination-initiating DNA double-strand breaks (DSBs) are repaired by crossovers or noncrossovers (gene conversions). While crossovers are easily detectable, noncrossover identification is hampered by the small size of their converted tracts and the necessity of sequence polymorphism. We report identification and characterization of a mouse chromosome-wide set of noncrossovers by next-generation sequencing of 10 mouse intersubspecific chromosome substitution strains. Based on 94 identified noncrossovers, we determined the mean length of a conversion tract to be 32 bp. The spatial chromosome-wide distribution of noncrossovers and crossovers significantly differed, although both sets overlapped the known hotspots of PRDM9-directed histone methylation and DNA DSBs, thus supporting their origin in the standard DSB repair pathway. A significant deficit of noncrossovers descending from asymmetric DSBs proved their proposed adverse effect on meiotic recombination and pointed to sister chromatids as an alternative template for their repair. The finding has implications for the molecular mechanism of hybrid sterility in mice from crosses between closely related Mus musculus musculus and Mus musculus domesticus subspecies. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2022 Elektronická adresa https://academic-oup-com.d360prx.biomed.cas.cz/genetics/article-abstract/217/1/iyaa013/5998651?redirectedFrom=fulltext
Počet záznamů: 1