Počet záznamů: 1
Biological autoluminescence as a noninvasive monitoring tool for chemical and physical modulation of oxidation in yeast cell culture
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SYSNO ASEP 0543278 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biological autoluminescence as a noninvasive monitoring tool for chemical and physical modulation of oxidation in yeast cell culture Tvůrce(i) Bereta, M. (SK)
Teplan, M. (SK)
Chafai, Djamel Eddine (URE-Y)
Radil, R. (SK)
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 4 Číslo článku 328 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova cell culture technique ; oxidation reduction reaction ; Saccharomyces cerevisiae Vědní obor RIV BO - Biofyzika Obor OECD Biophysics CEP GA18-23597S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora URE-Y - RVO:67985882 UT WOS 000627829300032 EID SCOPUS 85099207559 DOI https://doi.org/10.1038/s41598-020-79668-2 Anotace Normal or excessive oxidative metabolism in organisms is essential in physiological and pathophysiological processes, respectively. Therefore, monitoring of biological oxidative processes induced by the chemical or physical stimuli is nowadays of extreme importance due to the environment overloaded with various physicochemical factors. Current techniques typically require the addition of chemical labels or light illumination, which perturb the samples to be analyzed. Moreover, the current techniques are very demanding in terms of sample preparation and equipment. To alleviate these limitations, we propose a label-free monitoring tool of oxidation based on biological autoluminescence (BAL). We demonstrate this tool on Saccharomyces cerevisiae cell culture. We showed that BAL can be used to monitor chemical perturbation of yeast due to Fenton reagents initiated oxidation-the BAL intensity changes with hydrogen peroxide concentration in a dose-dependent manner. Furthermore, we also showed that BAL reflects the effects of low-frequency magnetic field on the yeast cell culture, where we observed a disturbance of the BAL kinetics in the exposed vs. control case. Our results contribute to the development of novel techniques for label-free, real-time, noninvasive monitoring of oxidative processes and approaches for their modulation Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2022 Elektronická adresa https://doi.org/10.1038/s41598-020-79668-2
Počet záznamů: 1