Počet záznamů: 1
CARD-FISH in the Sequencing Era: Opening a New Universe of Protistan Ecology
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SYSNO ASEP 0542712 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název CARD-FISH in the Sequencing Era: Opening a New Universe of Protistan Ecology Tvůrce(i) Piwosz, Kasia (MBU-M) ORCID
Mukherjee, Indranil (BC-A) RID, ORCID
Salcher, Michaela M. (BC-A) RID, ORCID
Grujcic, V. (SE)
Šimek, Karel (BC-A) RID, ORCIDČíslo článku 640066 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 12, MAR 4 2021 (2021)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova aquatic microbial food webs ; card-fish ; grazing by protists ; protists ; unicellular eukaryotes ; bacterivorous ; omnivorous and predatory flagellates ; heterotrophic and mixotrophic flagellates Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Mikrobiologie, virologie CEP GJ18-14095Y GA ČR - Grantová agentura ČR GX20-12496X GA ČR - Grantová agentura ČR GA19-23469S GA ČR - Grantová agentura ČR EF16_025/0007417 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA13-00243S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000629999400001 EID SCOPUS 85102799654 DOI 10.3389/fmicb.2021.640066 Anotace Phagotrophic protists are key players in aquatic food webs. Although sequencing-based studies have revealed their enormous diversity, ecological information on in situ abundance, feeding modes, grazing preferences, and growth rates of specific lineages can be reliably obtained only using microscopy-based molecular methods, such as Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). CARD-FISH is commonly applied to study prokaryotes, but less so to microbial eukaryotes. Application of this technique revealed that Paraphysomonas or Spumella-like chrysophytes, considered to be among the most prominent members of protistan communities in pelagic environments, are omnipresent but actually less abundant than expected, in contrast to little known groups such as heterotrophic cryptophyte lineages (e.g., CRY1), cercozoans, katablepharids, or the MAST lineages. Combination of CARD-FISH with tracer techniques and application of double CARD-FISH allow visualization of food vacuole contents of specific flagellate groups, thus considerably challenging our current, simplistic view that they are predominantly bacterivores. Experimental manipulations with natural communities revealed that larger flagellates are actually omnivores ingesting both prokaryotes and other protists. These new findings justify our proposition of an updated model of microbial food webs in pelagic environments, reflecting more authentically the complex trophic interactions and specific roles of flagellated protists, with inclusion of at least two additional trophic levels in the nanoplankton size fraction. Moreover, we provide a detailed CARD-FISH protocol for protists, exemplified on mixo- and heterotrophic nanoplanktonic flagellates, together with tips on probe design, a troubleshooting guide addressing most frequent obstacles, and an exhaustive list of published probes targeting protists. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2021.640066/full
Počet záznamů: 1